摘要
目的 了解离体皮肤冻伤模型创面愈合过程中,基质细胞衍生因子1（SDF-1）对创缘表皮干细胞（ESC）的运动趋化作用.方法 体外构建三维皮肤等价物全层冻伤模型（创面呈孔形）,免疫组织化学染色观察冻伤后3、7 d创缘间质内SDF-1的表达情况.另将冻伤模型分为对照组（创面区添加PBS 50 μL/孔）、SDF-1组（创面区添加100 ng/mL SDF-1,50μL/孔）和AMD3100组[创面区用100 ng/mL AMD3100（50μL/孔）处理30 min后,添加SDF-1 50μL/孔],观察各组创缘中ESC的分布变化.结果 免疫组织化学染色显示,冻伤后3、7 d创缘间质内SDF-1的表达逐渐增加.与对照组及AMD3100组相比,SDF-1组创缘基底层整合素β1阳性细胞数量增多,且部分阳性细胞向上层表皮迁移聚集,创缘ESC数量更多,表皮细胞层数增加.结论 SDF-1促进ESC向创缘迁移聚集参与创面修复,这可能是ESC参与创面修复过程的机制之一.
Objective To study the promotion effect of stromal cell-derived factor 1 （SDF-1） on the migration of epidermal stem cells （ESC） in the healing process of frostbite-wound model ex vivo. Methods A three-dimensional model of full-thickness frostbite of skin was constructed （with slot-like wound） out of skin equivalent. The expression of SDF-1 in wound stroma was observed with immunohisto-chemistry staining on post injury days （PID） 3 and 7. The model frostbite wounds were divided into control group （treated with PBS 50 μL per wound） , SDF-1 group （treated with 100 ng/mL SDF-1, 50 μL per wound） , and AMD3100 group [treated with 100 ng/mL AMD3100 （50 μL per wound） for 30 minutes, and then SDF-1 50 μL was added per wound]. The redistribution of ESC around wound was observed. Results The expression of SDF-1 in wound stroma increased gradually on PID 3 and 7. Compared with those in control and AMD3100 groups, there were more ESC and epithelial cell layers, and more integrin β1-positive cells appeared at the basal layer of wound in SDF-1 group, and some of the positive cells migrated upward to epidermis. Conclusions SDF-1 contributes to wound repair through promoting ESC to migrate toward and gather around wound edge. This may be one of the mechanisms of ESC participating in wound repair.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2010年第3期212-215,共4页
Chinese Journal of Burns
