摘要
目的探讨汉族基质金属蛋白酶-3（matrix metalloproteinase 3,MMP-3）-1171 5A/6A基因多态性与原发性高血压（essential hypertension,EH）伴颈动脉粥样硬化（carotid atherosclerosis,CAS）的关系。方法采用病例-对照研究,应用聚合酶链反应-限制性片断长度多态性（polymerase chain reaction？restriction fragmentlength polymorphism,PCR-RFLP）技术测定MMP-3-1171 5A/6A基因多态性,共检测了新疆医科大学第一附属医院高血压科住院的131例汉族EH伴CAS患者（essential hypertension with carotid atherosclerosis,AS组）和86例汉族EH伴颈动脉内膜正常患者（essential hypertension without carotid atherosclerosis,NS组）。结果 AS组的6A等位基因频率高于NS组（P〈0.05）,携带6A等位基因增加了EH伴CAS的危险度（OR=1.885,95%CI：1.013~3.505）。结论 MMP-3-1171 5A/6A基因多态性可能与汉族EH患者伴CAS有关,6A等位基因可能增加了EH患者伴发CAS的风险。
Objective To investigate the relationship between the single nucleotide polymorphisms of matrix metalloproteinase-3（MMP-3）-1171 5A/6A and essential hypertension with carotid atherosclerosis in Chinese Han population.Methods The study comprised 217 Han nationality participants,who were divided into two groups： essential hypertension with carotid atherosclerosis（AS group,n=131） and essential hypertension without carotid atherosclerosis（NS group,n=86）.Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies were determined.Results Individuals with 6A/6A genotype had 1.885 fold risk suffering from essential hypertension with carotid atherosclerosis,when compared with 5A/5A and 5A/6A genotypes.Conclusion The single nucleotide polymorphism of matrix metalloproteinase-3（MMP-3）-1171 5A/6A is probably associated with carotid atherosclerosis in essential hypertension people of Chinese Han population.
出处
《新疆医科大学学报》
CAS
2010年第4期365-368,共4页
Journal of Xinjiang Medical University
基金
乌鲁木齐市科学技术项目(Y05331001)
