摘要
从某化工厂的废水处理池污泥中分离到一株孔雀石绿降解菌,命名为K4-W.根据生理生化特征和16S rRNA基因序列相似性分析,将其初步鉴定为Raoultella sp.该菌在48h内对20mg/L的孔雀石绿降解率为82.5%,降解孔雀石绿的最适温度为30℃,外加碳源或Pb2+对其降解有明显的促进作用,增大接种量也有利于提高降解效率,较高浓度的孔雀石绿则对降解有抑制作用.该菌在降解过程中没有生成无色孔雀石绿,这不同于已报道的孔雀石绿降解途径,但该菌是否具有新的降解途径还需进一步研究.
A bacterial strain capable of degrading malachite green was isolated from the sludge sample collected from wastewater treating system of a chemical plant,and it was designated as strain K4-W.According to the similarity analysis ofits 16S rRNA gene sequence（GenBank Accession No.EU876828） and physiological-biochemical characteristics,it was preliminarily indentifi ed as Raoultella sp.Strain K4-W could degrade 82.5% of 20 mg/L malachite green within 48 h.The optimal temperature for the degradation was 30 ℃.The addition of Pb2＋ or other carbon sources could promote the degradation significantly.The increasing in inoculum also showed promoting effect on the degradation.The high concentration of malachite green would inhibit the degradation.No leucomalachite green appeared during the degradation process,which was different from the reported malachite green degrading pathway.Therefore,a further study was needed to verify whether strain K4-W harbored a new degradation pathway.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2010年第3期390-393,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家"863计划"项目(No.2007AA061101)
科技部自然科技资源平台项目(No.2005DKA21201-2)资助~~
