摘要
为了解甘蓝型油菜(Brassica napus)中磷酸烯醇式丙酮酸羧化酶(PEPC)基因的表达和分布情况,文章首先分析了油菜和拟南芥中PEPC基因之间的关系,同时参照拟南芥中4条PEPC基因扩增了针对甘蓝型油菜的探针,Southern blot结果表明,甘蓝型油菜中PEPC基因肯定多于4条,而RT-PCR分析了不同时期种子中的PEPC基因的表达情况,表明PEPC基因在种子成熟过程中油脂、储藏蛋白质等贮存物质的积累有一定作用。
The relationship of PEPC between Brassica napus and Arabidopsis thaliana was analyzed in order to know the background and the distribution expression of PEPC gene in Brassica napus.Meanwhile,probes were designed according to four PEPC genes of Arabidopsis thaliana.The Southern blot results showed that the number of PEPC genes in Brassica napus was certainly more than four.The RT-PCR analysis was carried out to investigate the expression of PEPC gene in seeds at different time.It was indicated that PEPC may have a certain role in accumulation of lipids and storage of proteins or other substances during the seed maturation process.
出处
《浙江农业学报》
CSCD
北大核心
2010年第3期276-280,共5页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(C020209)
浙江省自然科学基金(Y3090252)
