摘要
目的:研究不同添加剂对蛹虫草子实体生长分化的影响。方法:通过添加不同剂量的2,4一二氯苯氧乙酸(2,4—D)、玉米粉、柠檬酸三胺、秋水仙素和链霉素,分析其对蛹虫草生长分化及虫草素合成的影响。结果:在蛹虫草培养营养液中加有生长调节因子(2,4—D)(0.14g/L)和柠檬酸三胺(0.6g/L)的培养基,菌丝生长快,子实体分化率高,生长速度快、颜色深,且有较高的虫草素含量(0.46%);在培养基中加玉米粉,菌丝生长较快,但后期生长较慢;在培养基中加秋水仙素0.4g/L和链霉素0.8g/L,可诱导原基分化,增强培养基的抑菌能力,可提高产量和子实体中虫草素的含量。结论:在虫草培养营养液中加入生长调节因子(2,4—D)、柠檬酸三胺、秋水仙素和链霉素对提高蛹虫草子实体产量及虫草素含量有促进作用。
Objective： Study the growth and differentiation of fruitbody of cordyceps militaris （L.） Link with treatment additive Method： In this experiment, the micro additives were added in the culture medium to screen optimized additive for the high yield, well quality militaris. The compounds were a growth regulator 2,4-chlorophen-oxyacetic acid （2,4-D）, corn meal, citric acid triamine, colchicine, and streptomycin. The effect of those on the growth and development of cordyceps militaris was tested in the experiment. Result： The results showed that the growth of sino-canadian regulator （2,4-D） （0.14g/L） and citric acid triamine （0.6g/L） of the medium can have mycelial growth faster, fruithody differentiation higher, fruitbody growth speeder, frnitbody color deeper and cordycepin concentration higher （0.46%）. Corn flour takes mycelial growth faster but later and slower in the cuhivationr. Colchicine and streptomycin can induce primordium differentiation, and enhance the capacity of the medium to inhibit other bacterium, increase the yield of fruiting bodies and its cordycepin. Conclusion： the growth regulator （2, 4-D）, citric acid triamine, colchicine, streptomycin were added to nutrient solution in the cordyceps cultivation to improve the yield of cordyceps fruiting body and the cordycepin contentation.
出处
《食品与发酵科技》
CAS
2010年第3期60-64,共5页
Food and Fermentation Science & Technology
基金
四川省教育厅重点项目(No.08ZA086)
四川理工学院人才工程项目(07ZR15)
关键词
蛹虫草
虫草素
添加剂
产量
cordyceps militaris
cordycepin
optimized additive
output
