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重组人白细胞介素-6的纯化 被引量：1

Purification of recombinat human interleukin 6 expressed in E.coli
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摘要 重组人白细胞介素－６（ｒｈＩＬ－６）在工程菌ｐＢＶ２２０／ｒｈＩＬ－６／ＤＨ５ａ中以包涵体形式高效表达。ｒｈＩＬ－６经过工程菌体破碎、包涵体分离及抽提、复性、色谱分离后得到高度纯化。纯化产物纯度９５％。 rhIL 6 was highly expressed in E.coli in the form of inclusion bodies.rhIL 6 was pruified to homogeneity with cell disruption,inclusion bodies extraction and solubilization,renaturation and chromatography separation procedures.The purified protein showed good bioactivity in vitro and its purity is greater than 95%.
作者 储淳 周建 何黔川 刘国荣 刘社际 杨立明 刘波 彭如惠 帅凌燕
机构地区 卫生部成都生物制品研究所
出处 《微生物学免疫学进展》 1999年第1期47-50,共4页 Progress In Microbiology and Immunology
关键词 人白细胞介素-6 包涵体 复性 纯化 rhIL 6 Inclusion body Renature Purification
分类号 R331.142 [医药卫生—人体生理学] Q592.1 [生物学—生物化学]
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  • 1Kang J, Lemaire H G, Unterbeck A, et al. The precursor of Alzheimers disease amyloid A4 protein resembles a cell surface receptor. Nature, 1987, 325(6106): 733～736
  • 2Citron M, Diehl S T, Gordon G, et al. Evidence that the 42- and 40-amino acid forms of amyloid protein are generated form the amyloid precursor protein by different protease activities. Proc Natl Acad Sci USA, 1996,93(23):13170～13175
  • 3Selkoe D J. Cell biology of the amyloid protein precursor and the mechanism of Alzheimer possible form of preamyloid in Alzheimers disease. Ann Cell Boil,1994,10:373～403
  • 4Rossjohn J, Cappai R, Feil S C, et al. Crystal structure of the N-terminal, growth factor-like domain of Alzheimer amyloid precursor protein. Nature Structure Biol, 1999,6(4): 327～331
  • 5Surewicz W K, Epand R M. Role of peptide structure in lipid-peptide interactions: a fluorescence study of the binding of pentagastrin-related pentapeptides to phospholipid vesicles. Biochemistry, 1984,23(25): 6072～6077
  • 6Sreerama N, Venyaminov S Y, Woody R W. Analysis of protein circular dichroism spectra based on the tertiary structure classification. Anal Biochem, 2001,299(2): 271～274
  • 7Clarris H J, Cappai R, Heffernan D, et al. Identification of heparin-binding domains in the amyloid precursor protein of Alzheimers disease by deletion mutagenesis and peptide mapping. J Neurochem, 1997, 8(3): 1164～1172
  • 8Mok S S, Sberna G, Heffernan D, et al. Expression and analysis of heparin-binding regions of the amyloid precursor protein of Alzheimers disease. FEBS Lett, 1997, 415(3): 303-307
  • 9Clarris H J, Nurcombe V, Small D H, et al. Secretion of nerve growth factor from septum stimulates neurite outgrowth and release of the amyloid protein precursor of Alzheimers disease from hippocampal explants. J Neurosci Res, 1994,38(3): 248～258
  • 10Small D H, Nurcombe V, Reed G, et al. A heparin-binding domain in the amyloid protein precursor of Alzheimers disease is involved in the regulation of neurite outgrowth. J Neurosci, 1994, 14(4): 2117～2127

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微生物学免疫学进展
1999年 第1期

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