摘要
以磁性聚乙二醇为载体，通过吸附交联法固定化糖化酶．研究了戊二醛浓度、ｐＨ值及加酶量对酶固定化的影响．并对固定化酶的最适温度、最适ｐＨ、米氏常数、热稳定性及操作稳定性等进行了探讨．
A magnetic immobilized glucoamylase has been prepared by adsorbing glucoamylase onto a magnetic collidal particle and cross linking it with glutaraldehyde.The effects of different concentration of glutaraldehyde,pH of buffer,and the amount of glucoamylase on immobilization. The results showed that the activity of MIE was 17 095 U/g dry gel.The activity recovery was 63%. And some properties of magnetic immobilized and native glucoamylase such as optimum temperature、optimum pH and michaelis constant、stabilities to heat and operational stability were studied and compared.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第1期56-58,共3页
Progress In Biochemistry and Biophysics
基金
内蒙古自治区高等学校科学研究项目
关键词
葡萄糖淀粉酶
磁性聚乙二醇
磁性载体
固定化酶
glucoamylase, magnetic polyethylene particle, glutaldehyde, magnetic immobilized enzyme
