摘要
为了研究快速检测海产品中低浓度麻痹性贝毒(paralytic shellfish poisoning,PSP)的生化方法,在传统小白鼠法的基础上,使用浓度(以STXeq计)为1和0.2μg/kg的市售双壳贝类PSP提取液对小鼠进行腹腔注射,分别研究暴露15、60、120min后小鼠血液中ACh、AChE、NO和NOS的变化情况.结果发现,低浓度(0.2μg/kg)、短时间(15min)条件下仅ACh含量发生显著变化[与对照组相比p<0.05,含量(以蛋白计)为(141.2±14.8)μg/mg];而NO与NOS却在长时间(120min)暴露后才发生改变[与对照组相比p<0.05,分别为(68.7±3.8)μmol/g及(40.1±4.9)U/mg].在高浓度下,3个时间点的ACh含量也均发生显著变化.结果表明,在低浓度及短时间条件下反映PSP效应的指标仅是ACh,从而可以尝试选择其作为快速检测PSP的生化指标.
A rapid biochemical method was discussed in order to detect low concentration of paralytic shellfish poisoning （PSP） in sea food.The mice were injected with PSP extract of bivalves （1 and 0.2 μg /kg respectively,as STX equivalents） purchased from seafood market.ACh,AChE,NO and NOS in blood were studied at 15,60,120 min respectively.The results showed that at low dose （0.2 μg/kg） and 15 min,only the contents of ACh changed significantly compared with control group （p0.05）,which was （141.2 ± 14.8） μg /mg,while the contents of NO and the activities of NOS changed until 120 min,compared to control group （p0.05）,which were （68.7 ± 3.8） μmol /g and （40.1 ± 4.9） U /mg respectively.At high dose the contents of ACh changed at all three time point.It can be suggested that the contents of ACh is the only one of four indexes which can response to PSP at low dose in an early stage （15 min） and may be selected as a biochemical index for rapid detection of PSP.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2010年第7期1663-1666,共4页
Environmental Science
基金
上海科委科技攻关项目(08DZ1206302)
关键词
麻痹性贝毒
快速检测
生化指标
双壳贝类
paralytic shellfish poisoning（PSP）
rapid detection
biochemical index
bivalve
