摘要
目的探讨预先给予银杏叶提取物能否预防大鼠脊髓缺血再灌注损伤。方法将30只大鼠随机分为3组,假手术组(S组)、缺血再灌注组(IR组)、银杏叶提取物保护组(G组)。其中G组每日腹腔注射给予0.83 ml.kg-1GBE,连续给药5天。采用腹主动脉缺血法制备大鼠的脊髓缺血再灌注损伤模型。缺血45 min,再灌注24 h后进行Tarlov神经功能评价和病理组织学改变的观察。结果 G组与IR组相比较,神经功能评分结果显示IR组后肢神经功能改善明显,神经功能评分结果差异具有显著的统计学意义(P<0.05)。病理组织学结果表明,IR组出现明显的病理改变,神经细胞固缩、组织内血管充血等病理改变,G组的病理改变较轻,明显好于IR组。结论银杏叶对大鼠脊髓缺血再灌注损伤具有明显的保护作用。
Objective To discuss the protective effect of the ginkgo biloba extract （GBe） against ischemia-reperfusion injury of rat spinal cord. Methods 30 healthy rats were divided into three groups randomly： sham-operated group, Ischemia-reperfusion group, GBe group. In GBe group, 0.83 ml· kg^-1 GBE was administered by intraperitoneal injection per day for 5 days. Abdominal aorta occlusion was performed to prepare the rat spinal cord ischemia-reperfusion injury model. The stage of ischemia lasted 45 rain, and the stage of reperfusion lasted 24 h. Then neurological function was evaluated and pathohistological observation was performed. Results Neurological function results showed that neurological function of posterior limb of rats in GBe group was improved obviously and there was significantly different（ P 〈 0. 05）compared with IR group. Pathohistological changes such as pyknosis, paramorphia, vascular congestion and so on were observed in rats in IR group. But pathohistological changes in GBe group were improved, which was better than those in IR group. Conclusion Ginkgo biloba extract can prolect rats from spinal cord Ischemia- reperfusion injury.
出处
《中国实验诊断学》
北大核心
2010年第6期812-814,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技发展计划项目(200905183)
吉林省卫生厅科研基金项目(20082041)
关键词
银杏叶
脊髓
缺血再灌注损伤
保护
ginkgo biloba
spinal cord
ischemia-reperfusion injury
protection
