摘要
在0.02 mol/L NH4Cl-NH3.H2O(pH8.0)的底液中,采用循环伏安法测定葛根素,得到一良好的氧化峰,峰电位Ep=+0.57V,峰电流Ip与葛根素的浓度在1.046×10-7～5.767×10-5mol/L范围内成线性关系,相关系数r为0.9989,检出限为1.046×10-7mol/L.测定葛根中葛根素的含量,平均回收率在99.8%.并且研究了葛根素在玻碳电极上的电化学行为,结果表明葛根素的电极过程具有吸附性和不可逆性.
Puerarin shows a sensitive derivative oxidation peak at ＋0.57 V by using the cyclic voltammogram in a buffer solution of 0.02 mol/L NH4Cl-NH3·H2O（pH 8.0）.The peak current is proportional to the concentration of Puerarin in the range of 1.046×10-7～5.767×10-5 mol/L with r =0.9989.The detection limit is 1.046×10-7mol/L.This method has been applied to determine Puerarin with satisfactory results.Its average recovery rate is 99.8%.The electrochemical behavior of the system has been studied at a glassy carbon electrode by cyclic voltammetry.The electrode oxidation process of Puerarin at the glassy carbon electrode is irreversible and adsorptive.
出处
《分析测试技术与仪器》
CAS
2010年第2期97-99,共3页
Analysis and Testing Technology and Instruments
关键词
葛根素
循环伏安法
puerarin
cyclic voltammetry
