摘要
膜蛋白是连接细胞与外界环境的重要载体,发挥着物质转运、信号传导等作用。通过对一种新分离出来的中度噬盐菌Bacillussp.Ⅰ121进行了质膜蛋白质组学分析,盐胁迫下该菌株主要靠积累脯氨酸来维持细胞渗透压。在对Bacillussp.Ⅰ121在不同盐浓度下的生长情况进行测定的基础上,我们选取了1%,5%,10%,15%(w/v)NaCl4个盐浓度进行细胞培养,并分别提取细菌的质膜,通过5%～20%的梯度凝胶SDS-PAGE电泳将疏水的膜蛋白进行分离,随后对盐胁迫响应的8条不同的蛋白条带进行了ESI-LC-MS/MS质谱分析。将所得结果在NCBI数据库中进行比对,最终成功鉴定出10种蛋白。这些蛋白涉及物质跨膜转运,膜的生物合成以及胁迫信号转导等功能。为膜蛋白在盐胁迫响应过程中的作用方面的研究开辟了新思路。
Membrane proteins are essential molecules that interface the cells with environment,and are responsible for membrane transport,signal transduction.In this study,a membrane proteomic analysis of the novel halophilic bacterium Bacillus sp.Ⅰ121 was carried out.Identification of compatible solutes indicated that Ⅰ121 mainly accumulate proline to counteract salinity.Upon the growth curves of the strain Ⅰ121 under different salinity,membrane fractions were prepared from the cells cultured differently at 1%,5%,10% and 15%（w/v） NaCl concentrations.The hydrophobic membrane proteins were separated by 5%-20% gradient SDS-PAGE.Eight differential stained bands corresponding to NaCl concentration were analyzed by ESI-LC-MS/MS.The results were searched against NCBI database and 10 proteins were identified.These proteins involved in membrane transport,membrane biogenesis,and stress signal molecules.The research provides insights into the functions of membrane proteins responsible for coping with high salinity.
出处
《生物学杂志》
CAS
CSCD
2010年第3期1-4,共4页
Journal of Biology
基金
国家自然科学基金(30400030)
关键词
噬盐菌
盐胁迫
膜蛋白
蛋白质组学
halophilic bacterium
salt stress
membrane proteins
proteomics
