摘要
棉籽粕经脱棉酚处理后,不经过提取棉籽蛋白的中间步骤,直接利用碱性蛋白酶酶解制备棉籽多肽。在单因素实验的基础上,选取酶解温度、酶解pH、加酶量、酶解时间为影响因素,应用响应面法的Box-Behnken中心组合实验进行设计,以棉籽多肽产率为响应值,对制备条件进一步优化。结果表明,采用响应面法得到的最佳制备工艺条件为:酶解温度56.4℃,酶解pH 9.7,加酶量8.4%,酶解时间4.1 h,底物浓度3%,此时的棉籽多肽产率为49.60%。
Preparation of peptides from degossypoled cottonseed meal by basic protease hydrolysis was studied without the process of extracting the cottonseed protein.Based on single-factor experiments,four independent variables,namely enzymolysis temperature,pH,enzyme dosage and enzymolysis time were selected as influence factors,and the Box-Behnken central composite experiment design was used to optimize the preparation conditions of peptides.The optimal conditions were determined as follows：enzymolysis temperature 56.4℃,pH 9.7,enzymolysis time 4.1 h,substrate concentration 3%,enzyme dosage 8.4%.Under the optimal conditions,the yield of cottonseed peptides was 49.60%.
出处
《中国油脂》
CAS
CSCD
北大核心
2010年第6期22-26,共5页
China Oils and Fats
基金
国家“十五”重大科技专项资助项目(2001BA201A20)
湖北省重大科技攻关项目(2006AA201B32)
关键词
多肽
制备
脱棉酚
响应面法
peptides
preparation
degossypoling
response surface methodology
