摘要
目的：研究通痹灵（ＴＢＬ）对佐剂性关节炎（ＡＡ）大鼠滑膜成纤维细胞增殖及滑膜细胞分泌白细胞介素１（ＩＬ１）、肿瘤坏死因子α（ＴＮＦα）和前列腺素Ｅ２（ＰＧＥ２）的影响。方法：采用组织块培养的方法获得滑膜成纤维细胞，检测原代培养大鼠滑膜细胞培养上清对滑膜成纤维细胞增殖反应的影响，以及滑膜细胞培养上清中ＩＬ１、ＴＮＦα的活性和ＰＧＥ２的含量。结果：ＴＢＬ可以明显抑制ＡＡ大鼠滑膜成纤维细胞增殖（Ｐ＜０００１），下调ＡＡ大鼠滑膜细胞分泌ＩＬ１、ＴＮＦα和ＰＧＥ２（Ｐ＜００１）；消炎痛对滑膜成纤维细胞的增殖具有明显的刺激作用（Ｐ＜０００１），虽能显著抑制ＡＡ大鼠滑膜细胞分泌ＰＧＥ２，但进一步上调了ＩＬ１、ＴＮＦα的产生（Ｐ＜００１）。结论：ＴＢＬ治疗ＲＡ的机理之一可能是通过下调滑膜细胞的分泌功能。
Objective: To study the effect of Tongbiling (TBL) on the
proliferation of synovial fibroblast and interleukin 1 (IL 1), tumor necrosis factor α (TNF α)
and postaglandin E 2 (PGE 2) secretion by synoviocytes in adjuvant arthritis (AA) rats.
Methods: Synovial fibroblasts was derived from culture of tissue piece. The effect of primary
synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL 1, TNF α
bioactivity and PGE 2 content of supernatants of cultured synoviocytes were measured.
Results: TBL could inhibit significantly the synovial fibroblast proliferation (P<0 001), and
down regulated IL 1, TNF α and PGE 2 productions (P<0 01); indomethacin could promote
obviously the synovial fibroblast proliferation (P<0 001). It inhibited significantly PGE 2
production, but further up regulated the IL 1 and TNF α secretion by synoviocytes (P<0 01).
Conclusion: The therapeutical effect of TBL on AA might be associated with its down
regulating the secretory function of synoviocyte, then restoring the abnormal proliferation of
fibroblast to normal levels.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1999年第3期167-169,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省自然科学基金
关键词
关节炎
佐剂性关节炎
滑膜
中医药疗法
通痹灵
adjuvant arthritis, synovialmembrane, cell culture,
fibroblast, cyto proliferation, cytokine, Tongbiling
