摘要
目的确定不同型人乳头瘤病毒（ＨＰＶ）感染的自然历程以及其持续感染在子宫颈癌发展过程中的作用。方法应用聚合酶链反应检测荷兰１５５名妇女子宫刮颈片中的ＨＰＶＤＮＡ，应用线样探针分析法（ＬｉＰＡ）进行，包括ＨＰＶ６，１１，１６，１８，３１，３３，３５，４０，４２，４３，４４，４５，５１，５２，５６和５８的基因分型。结果１５５例妇女子宫颈刮片中ＨＰＶＤＮＡ检出率为６０％，其中在宫颈细胞学检查正常或仅有炎症病变的妇女中ＨＰＶＤＮＡ阳性率为４３％，而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为８１．２％。９３例ＨＰＶ阳性的标本中，８９例利用ＬｉＰＡ成功地作出了基因分型，其中单独一型ＨＰＶ感染为４９例，涉及１１种不同基因型ＨＰＶ，最常见的型别为ＨＰＶ１６，占单独感染的５１％（２５／４９）；另４０例为不同型别ＨＰＶ重叠感染，涉及所有１６种ＨＰＶ基因型别，二重感染占主导地位（ｎ＝２５），同时也检测出其它多重感染。４例ＨＰＶ阳性的标本应用ＬｉＰＡ未能作出基因分型，ＤＮＡ序列分析显示这４例为ＨＰＶ６６感染，此型别不包括在ＬｉＰＡ所标记的探针内。１２例随诊资料显示了ＬｉＰＡ的物异性及高度的重复性。结论线样探针分析法可以用于子宫颈?
Objective To pursue the further development of a practical HPV genotyping system applicable for mass screening. Methods Cervical scrapes from 155 women were tested for the presence of HPV using a general primer based PCR. A reverse hybridization assayor, the line probe assay (LiPA) was used for genotyping 16 different HPV types(HPV 6, 11, 16, 18, 31, 33, 35, 40, 42, 43, 44, 45, 51, 52, 56, 58)simultaneously. Results 93(60%) out of 155 specimens tested were positive for HPV DNA by PCR. The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV 16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections( n =25) prevailed, triple( n =9),quadruple( n =3) and quintuple( n =3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV 66 which was not included in LiPA probes. Follow up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform. It allows accurate and rapid identification of 16 different HPV types in cervical scrapes and will facilitate HPVdetection as well as genotyping in cervical cancer screening programs.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1999年第1期9-12,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
人乳头瘤病毒
聚合酶链反应
基因分型
Human papillomavirus Polymerase chain reaction Line probe assay Genotyping
