摘要
目的观察白屈菜红碱对四氯化碳诱导肝纤维化大鼠血清谷丙转氨酶(ALT)和透明质酸(HA)的影响。方法用四氯化碳皮下注射,同时联合营养控制和饮用10%酒精复合法制备SD大鼠肝纤维化模型,在实验第4周末,肝纤维化模型建立(2期肝纤维化形成),然后用不同剂量白屈菜红碱治疗,同时设立病理模型组、正常对照组和IFN-γ组。给药8周末取血清检查HA、ALT。结果白屈菜红碱各剂量组血清HA水平较模型组显著降低(P<0.05),与IFN-γ组比较无明显差异(P>0.05);血清ALT在白屈菜红碱各剂量组、IFN-γ组与模型组之间变化无明显差异(P>0.05)。结论白屈菜红碱能降低血清HA的含量,具有抗化学性大鼠肝纤维化作用,且疗效与IFN-γ相当;但白屈菜红碱没有降低血清ALT,无保护肝细胞的作用。
Objective To observe the curative effects of chelerythrine on the serum ALT and HA level of CCl4-induced hepatic fibrosis in rats.Methods Models of hepatic fibrosis were established by hypodermic injection of tetrachloride,in combination with the control of nutrition and the drinking of 10% alcohol to rats.According to histological sections,hepatic fibrosis in rats emerged at the end of the fourth week.Subsequently different doses of chelerythrine was used to treat hepatic fibrosis in rats.In addition,normal control group,fibrotic model group,γ-interfom group in experiment was arranged.At the end of the eighth week of treatment,serum hyaluronic acid level,alanine aminotransferase of all the rats were detected.Results Compared with fibrotic model group,all chelerythrine groups could decreased significantly the serum HA level of hepatic fibrosis in rats induced by CCL4（P0.01）,which was not obviously distinguished from γ-interfom group（P0.05）,while the serum ALT level of hepatic fibrosis in rats was not obviously different among all chelerythrine groups,IFN-γ group and fibrotic model group.Conclusions Chelerythrine can reduce the serum HA level of hepatic fibrosis,and the anti-fibrotic effects of chelerythine are identical with IFN-γ.But chelerythine could not improve the serum ALT level and protect live cell of hepatic fibrosis in rats.
出处
《南华大学学报（医学版）》
2010年第3期325-327,共3页
Journal of Nanhua University(Medical Edition)
