摘要
目的:研究银杏黄酮磷脂复合物预处理对大鼠局灶性脑缺血再灌注损伤后脑组织中NF-κB和一氧化氮(NO)水平的影响,并探讨其发生的可能机制。方法:线栓法建立右侧大脑中动脉闭塞大鼠模型,分别于缺血90min后再灌注6h、24h、72h。缺血前1h及随后每6h灌胃给药。测定脑组织中的NO水平。免疫组织化学方法检测脑组织内NF-κB的表达。结果:NF-κB明确表达于脑缺血再灌注组手术侧半球的海马及皮质,NO水平明显升高。银杏黄酮磷脂复合物可显著降低缺血再灌注损伤大鼠脑组织NF-κB的表达和NO含量。结论:银杏黄酮磷脂复合物预处理可以通过降低脑缺血再灌注损伤后脑组织中NO水平和NF-κB的表达而起到神经保护作用。
Objectiveb：To observe the effect of ginkgo flavone phytosomes on the experimental cerebral ischemic reperfusion injury in rats and its possible mechanisms,including NO and expression of NF- κ B in rat aorta.Methods： The models were established by an intraluminal suture method to induce right middle cerebral artery occlusion in rats. After cerebral ischemia for 90 minutes followed by reperfusion for 6h,24h,72h, Rats were given drug before 1 hour of model replicating. After model replicating drug were fed every 6 hours. Reperfusion was carried on after 90 minutes of cerebral ischemia in model group and treatment group. Rats were decapitated for preparing brain tissue in reperfusion of 6h,24h and 72h.NO was detected.The expression of NF- κ B were assayed by immunohistochemical staining. Results： NF- κB positive reaction increased obviously in the hippocampi,cortex and medulla. The level of NF-κB and NO can be decreased by ginkgo flavone phytosomes obviously. Conclusion： NF- κB can be decreased by ginkgo flavone phytosomes,and it can decrease NO,this may be one of the mechanisms of ginkgo flavone phytosomes neur-pretection.
出处
《中国西部科技》
2010年第16期40-41,50,共3页
Science and Technology of West China
