摘要
采集云南宣威市肺癌高发区虎头村和肺癌低发区宁家湾村冬季室内PM10样品,利用肺上皮细胞A549建立颗粒物染毒模型,以AnnexinV-FITC/PI双染色流式细胞分析法(FCM)检测PM10染毒后的细胞凋亡情况.AnnexinV-FITC/PI流式细胞分析结果显示,在相同浓度PM10作用下虎头村组肺泡上皮细胞凋亡率、死亡率均比宁家湾组高.宣威肺癌高发区虎头村组室内PM10对人肺泡上皮细胞凋亡的影响大于对照点宁家湾村.
Indoor PM10 was collected from Hutou village（high incidence area of lung cancer） and Ningjiawan village（low incidence area of lung cancer） respectively in Xuanwei Town;the model of cell apoptosis was set up by exposing the lung epithelial cells A549 to PM10,then detect the cell apoptosis by Annexin-V FITC/PI flow cytometry（FCM）.Comparison of the two groups suggested that the total apoptosis rates and the mortality rates of Hutou Group increased significantly.The impact on lung cell apoptosis of indoor PM10 collected form Hutou village was larger than that from Ninjiawang village.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2010年第7期1004-1008,共5页
China Environmental Science
基金
国家自然科学基金资助项目(40672103)
教育部科技创新工程重大项目培育资金资助项目(705022)
