摘要
目的:研究月腺大戟根中总黄酮类化合物的提取工艺,为研发新型植物源农药提供理论基础。方法:采用索氏提取法对月腺大戟根总黄酮进行提取,以乙醇体积分数、提取时间、提取温度、料液比为实验因素,以提取的总黄酮含量为指标,对提取工艺进行考察,确定最佳提取条件。结果和结论:根总黄酮提取的优化条件为:提取时间2 h,提取温度70℃,料液比1:40,乙醇浓度50%;影响提取的主次顺序为:料液比>提取时间>乙醇浓度>提取温度;其中料液比和提取时间是影响根总黄酮提取效果的显著因素。
Objective： To study the extraction process of total flavones from the roots of Euphorbiae bracteolata Hayata, and accordingly provide evidence for research and development of botanical pesticide. Method ： The total flavones were extracted by Soxhlet way, The experimental factors include： concentration of ethanol, time and temperature of extracting, ratio of material to fluid. The extraction conditions were determined by the content of total flavones. Result and conclusion ： The optimal conditions on total flavones extracted of Euphorbiae bracteolata Hayata were as follows ： time of extracting is 2 h, temperature of extracting is 70 ~C , ratio of material to fluid is 1 ： 40 and concentration of ethanol is 50 %. The order on purification is ratio of material to fluid 〉 time of extracting 〉 concentration of ethanol 〉 temperature of extracting and the significantly factors are ratio of material to fluid and time of extracting.
出处
《激光生物学报》
CAS
CSCD
2010年第3期413-417,共5页
Acta Laser Biology Sinica
关键词
月腺大戟
总黄酮
正交实验
提取工艺
Euphorbiae bracteolata Hayata
total flavones
orthogonal design
extraction process
