摘要
目的分析预测马铃薯卷叶病毒(PLRV)外壳蛋白的理化性质及结构。方法应用DNA-MAN软件、ExPASy中的ProtParam工具,SignalP,LipoP程序,TMHMM,PSIPRED和3D-PSSM等方法,分析PLRV.CP的核苷酸基本特征、蛋白质理化性质、亲疏水性、可溶性、信号肽、跨膜结构域、二级结构、保守区域、三级结构等。结果陕西PLRV.CP,与已报道的PLRV.CP高度同源。蛋白序列分析显示无跨膜螺旋结构,不属分泌蛋白,是可溶的亲水性蛋白。二级结构主要为折叠和无规则卷曲,不含转角。三级结构保守区预测表明,该蛋白属于黄化病毒属外壳蛋白成员。结论对马铃薯卷叶病毒外壳蛋白的生物信息学分析为进一步研究其功能、探索抗病途径奠定了基础。
Aim To analyze and predict the function and physical and chemical properties of coat protein（ CP） of Potato Leafroll Virus（ PLRV）. Methods The CP was analysed through DNAMAN,ProtParam,SignalP3. 0,LipoP1. 0,TMHMM-2. 0,PSIPRED and 3D-PSSM about the nucleotide properties,physical and chemical properties,hydrophilic and hydrophobic,solubility signal peptide,transmembrane,secondary structure,conserved domain and tertiary structure. Results CP from Shaanxi PLRV has a high homology with other reported CP of PLRV. Protein sequence analysis showed that the CP of Shaanxi PLRV has no transmembrance domain,it＇s a non-translocation and water soluble protein. Analysis of the CP protein structure showed that the secondary structure mainly contains helix and coil,but without turn. The conservative domain analysis showed that the PLRV.CP belong to the coat protein of luteovirus. Conclusion The bioinformatics analysis on PLRV.CP lay the root for function study and pathways in virus resistance.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期473-476,共4页
Journal of Northwest University（Natural Science Edition）
基金
西北大学国家大学生创新性实验计划基金资助项目(091069702)
