摘要
目的探讨不同条件制备冷冻血小板质量的影响因素并分析其原因。方法冷冻血小板融化后室内温度下放置于玻璃或瓷砖冷面介质、导热慢的木板或毛巾等介质和(22±2)℃震荡介质中保存,观察冷冻血小板融化复苏后纤维蛋白及絮状不可逆聚集物析出的情况以及冷冻的温度和不同速冻方式对血小板质量的影响。结果冷冻血小板融化后放置于导热性能良好介质,保存血小板纤维蛋白及絮状不可逆聚集率为100%,导热慢的木板或毛巾介质和(22±2)℃震荡保存血小板纤维蛋白及絮状不可逆聚集率为0%,冻结与解冻温差不同,纤维蛋白及絮状不可逆聚集的发生率差异有统计学意义(x2=547.69,P<0.01)。结论冷冻血小板融化后于(22±2)℃震荡保存冷冻血小板质量更好,所有过程均不可放置冷面介质暂存。
Objective To study the effect of different conditions on the quality of frozen platelets. Methods Frozen platelets were stored in different medium, at glass or ceramic tile, and heat conduction slow plank or towel, and （22 ± 2） ℃, respectively. The formation of fibrin and irreversible aggregation were obsered in these melt resuscitative frozen platelets. Results The rate of fibrin and irreversible aggregation was 100% when ptatelets were stored at glass or ceramic tile. The rate of fibrin and irreversible aggregation was 0% when platelets were stored at （22 ±2）℃ and heat conduction slow plank or towel. In different medium, the formation of fibrin and irreversible aggregation was significant different （ x2 = 547. 69 P 〈 0.01 ）. The maintain temperature and the frozen mode in platelets could affect the quality of platelets. Conclusion Platelets stored at （22 ± 2） ℃ could improve the stability of melt resuscitative frozen platelets by preservation the labile coagulation factors in platelets.
出处
《海南医学》
CAS
2010年第13期27-29,共3页
Hainan Medical Journal
