摘要
目的:探讨葡天胶囊预处理对大鼠肾脏缺血再灌注损伤的保护作用。方法:雄性Wistar大鼠24只,随机分为假手术组(Sham组)、模型对照组(Control组)、葡天胶囊预处理组(PT组),每组各8只。模型对照组及葡天胶囊160mg.kg-1.d-1预处理组手术建立肾脏缺血再灌注模型,缺血后1h恢复灌注,分别在恢复灌注后24h、48h、72h后检测血肌酐、尿素氮及血清白蛋白水平,并观察肾脏病理变化。结果:与假手术组相比,模型组血肌酐水平升高(P<0.05);葡天胶囊预处理组再灌24h、48h及72h血肌酐水平明显低于模型组(P<0.05);肾脏病理显示葡天胶囊预处理组肾组织病变轻于模型对照组。结论:葡天胶囊对肾脏缺血再灌注损伤有保护作用,具有一定的临床应用价值。
Objective：To explore the protective effects of Putian capsule on renal ischemia reperfusion injury（I/R）rats models.Methods：Twenty-four Wistar male rats were divided evenly into 3 groups randomly：the Sham-operated group（group Sham）,the renal ischemia reperfusion injury group（group I/R）and the Putian preconditioning group（group PT）.The rats in group I/R and PT were made I/R models by operation and the ischemia time was 1 hour.The PT group were treated by PT 160 mg·kg^-1·d^-1 before the operation.The levels of serum creatinine（Scr）,blood urea nitrogen（BUN）and albumin（Alb）were detected respectively at 24,48 and 72 hour after ischemia and reperfusion（I/R）happened.Pathological changes in the different time were also noted by light microscope.Results：Compared with those in the sham group,the Scr and BUN were much higher in the control group after I/R at 24,48 and 72 hour,while these were not significantly elevated in PT group.The renal pathological changes were more severe in the model group than that of PT group.Conclusion：The results demonstrated that Putian capsule could protect the renal function against renal ischemia reperfusion injury and it could be used as a valuable clinical product.
出处
《中国中西医结合肾病杂志》
2010年第4期295-297,I0002,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
全军医药卫生科研基金资助项目(No.06MA265)
关键词
葡天胶囊
肾脏缺血再灌注损伤
大鼠
Pu Tian capsule Renal ischemia reperfusion injury（I/R）Rats
