摘要
目的:探讨缺血性脑卒中大鼠小肠动力的改变及与血清Ghrelin变化的关系.方法:48只♂Wistar大鼠随机分为对照组(n=8)和实验组(n=40),实验组按照3、6、12、24、48h随机分为5个亚组,每组大鼠8只.采用Longa线栓法建立大脑中动脉闭塞(middlecerebral artery occlusion,MCAO)模型(对照组组大鼠仅切开皮肤,不放线栓),所有大鼠进行神经生物学评分,MCAO组进行脑组织TTC染色.美蓝染色法检测肠动力;酶联免疫法测定血清Ghrelin水平;取距回盲部5cm处小肠,光镜及电镜下观察肠黏膜组织的变化.结果:对照组大鼠神经生物评分为0分,MCAO组大鼠神经生物评分为1-3分,MCAO组大鼠脑组织TTC染色可见缺血坏死区.MCAO各亚组的小肠推进比均明显低于对照组(P<0.05),脑缺血后24h降至最低.MCAO各亚组血清Ghrelin水平均明显高于对照组(P<0.05),血清Ghrelin水平于术后3h即升高,24h达峰值.相关性分析显示缺血性脑卒中大鼠血清Ghrelin水平与小肠推进比之间呈线性关系(r=-0.841,P<0.05).光镜下MCAO组肠黏膜上皮细胞受损,电镜下可见大量线粒体肿胀、嵴断裂,细胞器空泡变性,细胞间紧密连接偶见增宽,可见凋亡细胞.结论:MCAO大鼠局灶性脑缺血后出现肠动力改变、血清Ghrelin水平变化,脑缺血24h内小肠动力显著下降,考虑可能与脑缺血应激后多种因素共同作用的结果,其机制仍需进一步研究.
AIM：To determine the changes in serum ghrelin and small intestinal motility in rats with cerebral arterial thrombosis and to explore their relationship. METHODS：Forty-eight Wistar rats were randomly divided into two groups：control group （n = 8） and middle cerebral artery occlusion （MCAO） group （n = 40）. The MCAO group was further equally divided into 5 subgroups for testing at 3,6,12,24 and 48 h after cerebral arterial thrombosis. MCAO was induced in rats using the thread occlusion method proposed by Longa. Neurologic examinations were conducted and neurologic findings were scored at each time point. Triphenyltetrazolium chloride （TTC） staining of brain tissue was performed. The content of serum ghrelin was determined by enzyme-linked immunosorbent assay,and small intestinal motility was measured by methylene blue staining. The ileum tissue was examined by light and electron microscopy. RESULTS：The neurologic scores were 0 for all the rats in the control group and 1-3 for those in the MCAO group. Small intestinal motility was significantly lower in the MCAO group than in the control group （P 0.05）,reaching the lowest level at 24 h. Serum ghrelin level increased at 3 h and reached the peak at 24 h. Serum ghrelin level was significantly higher in the MCAO group than in the control group （P 0.05）. Correlation analysis showed a linear relationship between serum ghrelin levels and small intestinal motility （r = -0.841,P 0.05） in rats with MCAO. Intestinal mucosal injury could be seen by light and electron microscopy,such as intestinal villus swelling,chondrosome swelling,vacuolar degeneration,apoptosis,etc. CONCLUSION：Changes in serum ghrelin and small intestinal motility occur in rats with MCAO. The change of intestinal motility is significant at 24 h after cerebral ischemia.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第15期1550-1554,共5页
World Chinese Journal of Digestology
基金
首都医学发展科研基金资助项目
No.2007-3098~~
