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SiRNA-EGFR对人乳腺癌细胞表皮生长因子受体表达的影响 被引量：1

Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells
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摘要 目的探讨SiRNA-EGFR对人乳腺癌细胞乳腺癌表皮生长因子受体表达的影响。方法应用RT-PCR测定RNA干扰人乳腺癌细胞MDA-MB-231、MDA-MB-453S、ZR-75和ZR-75-30后Hyase基因的mRNA的表达变化。结果 SiRNA-EGFR作用于表达不同水平透明质酸酶的人乳腺癌细胞株MDA-MB-231、MDA-MB-453S、ZR-75-30和ZR-75后能明显抑制其乳腺癌表皮生长因子受体的mRNA表达水平(P<0.05)。结论 SiRNA-EGFR作用于人乳腺癌细胞后能特异性抑制EGFR的mRNA的表达水平。 Objective To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells.Methods Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231,MDA-MB-435S,ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR.Results After transfection with SiRNA-EGFR,the expression levels of EGFR were significantly inhibited in MDA-MB-231,MDA-MB-435S,ZR-75 and ZR-75-30 cells（P0.05）.Conclusion Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.
作者 吴伟东 方驰华 杨正心 包佳巾
机构地区 广州市红十字会医院胸外科 南方医科大学珠江医院外科
出处 《南方医科大学学报》 CAS CSCD 北大核心 2010年第6期1260-1263,共4页 Journal of Southern Medical University
基金 广东省科技成果推广项目(2004A30202020003)
关键词 人乳腺癌细胞 RNA干扰 表皮生长因子受体 human breast cancer cells RNA interference epidermal growth factor receptor
分类号 R737.9 [医药卫生—肿瘤]
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参考文献12

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共引文献5

同被引文献16

  • 1廖小金.表皮生长因子结构和生物学效应[J].海峡药学,2006,18(5):14-17. 被引量：21
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  • 9许民,王建中,刘朝霞.6例子宫内膜癌复发临床分析[J].中国现代医生,2008,46(34):148-149. 被引量：5
  • 10李淑霞,陈嘉薇,杨兆瑞,路光中,唐华美,胡宏慧.分化相关基因1与雌激素受体α在Ⅰ型子宫内膜癌中表达的相关性分析[J].上海医学,2008,31(12):877-879. 被引量：2

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二级引证文献2

南方医科大学学报
2010年 第6期

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