摘要
用RAPD标记对1份来自上海和45份来自西南地区（四川、重庆、云南、贵州）的扁穗牛鞭草无性系的遗传多样性进行检测。研究结果表明,①14条引物共扩增出198条谱带,平均每条引物扩增出14.1条带,多态性比率（PPB）达91.9%,多态信息含量（PIC）范围为0.208~0.313,材料间遗传相似系数（GS）在0.624~0.973之间,表现出丰富的遗传多样性。②对RAPD分子标记扩增条带数据进行聚类,能将高大直立型和低矮型的材料分开,可能是因为所检测到的RAPD标记中存在与形态性状密切相关的数量性状位点（QTL）,这为进一步应用RAPD分子标记对扁穗牛鞭草群体基因组进行扫描,寻找与目标寻找紧密连锁的分子标记提供了理论基础。
RAPD molecular marker was applied to detect genetic variation of 46 Hemarthria compressa clones from Shanghai and Southwest China,including Sichuan,Chongqing,Yunnan and Guizhou.（i） 14 primers were selected for RAPD analysis from 100 random primers,of which amplification bands were clear.198 DNA fragments were found with an average of 14.1 bands per primer.The percentage of polymorphism bands（PPB） was 91.9 %,the polymorphism information content（PIC） ranged from 0.208 to 0.313 with an average of 0.258,the genetic similarity coefficient（GS） among all clones ranged from 0.624 to 0.973,which indicated that the genetic diversity was comparatively rich among the clones tested.（ii）The Hemarthria compressa materials with high-horrent and thickset-low were distinguished through UPGMA cluster.The reason may be that there were quantitative trait loci among RAPD markers detected which were associated closely with morphical trait,that provided a basis for scaning Hemarthria compressa population genome by RAPD marker to find molecular marker closely linked to target traits.These result provided useful information for germplasm storage,utilization and breeding.
出处
《西南农业学报》
CSCD
北大核心
2010年第3期866-871,共6页
Southwest China Journal of Agricultural Sciences
基金
国家"973"基础研究项目(2007CB108907)
