摘要
目的研究异丙肾上腺素(ISO)致大鼠心肌肥厚中PI3K和Akt在心肌组织中的表达,为探讨心肌肥厚的信号转导机制和逆转心肌肥厚提供形态学资料。方法健康成年SD大鼠20只,随机分为实验组、对照组,每组10只。实验组给予异丙肾上腺素处理。1周后处死大鼠,取心肌组织,常规石蜡切片,HE染色,观察心肌组织的病理变化,测量心肌肥厚指标;免疫组织化学染色和免疫荧光染色,检测p-PI3K和p-Akt的表达及分布。结果实验组大鼠心肌肥厚指标与对照组相比均明显升高;免疫组织化学检测显示,实验组心肌组织p-PI3K和p-Akt蛋白表达面积和平均光密度较对照组高。免疫荧光检测实验组心肌组织p-PI3K和p-Akt蛋白表达较对照组高。结论小剂量持续给予ISO能建立大鼠心肌肥厚模型;p-PI3K和p-Akt蛋白表达均与心肌肥厚的发生和发展过程相关,PI3K/Akt信号通路激活,可能是导致心肌肥厚的机制之一。
Objective To provide morphological data for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy by researching the expression of PI3 K and Akt in cardiac tissue in isoproterenol-induced myocardial hypertrophy. Methods Twenty healthy adult SD rats were randomly allocated into 2 groups ： experimental group （n =20） and control group （n = 10）. The hearts were taken from rats after a week, paraffin sections of rat myocardial tissue were made in order to stain by HE for pathological analysis and measure the contents of index of myocardial hypertrophy; The expression and distribution of p-PI3K, p-Akt protein in the experimental group and control group were detected by immunohistochemistry and immunofluorescence. Results Index of myocardial hypertrophy in the experimental group were significantly higher than those of the control group; the results of immunohistochemical detection showed that in protein expressions area, average optical density of p-PI3K, p-Akt in the experimental group were significantly higher than those in the control group. Compared with the control group, the positive expression of p-PI3K and p-Akt in the experimental group was significantly increased. Conclusion A model of cardiac hypertrophy can constructed by the method of continually injecting low-dose ISO to rats. p-PI3K and p-Akt protein expression are related to the occurrence of cardiac hypertrophy and relevant development; the PI3K/Akt signaling pathway activation could be one of the mechanisms of cardiac hypertrophy.
出处
《四川动物》
CSCD
北大核心
2010年第4期560-562,F0004,共4页
Sichuan Journal of Zoology
基金
河南省科技厅07年立项课题(项目编号:072300450330)
关键词
PI3K
AKT
心肌肥厚
免疫组化
免疫荧光
PI3K
Akt
immunohistochemistry
immunofluorescence
cardiachypertrophy
