摘要
通过现场取样、显微镜检、细菌培养、组织病理、电镜观察以及PCR检测等研究手段,对2008年9月浙江省舟山市普陀区某梭子蟹养殖塘混养的脊尾白虾"白浊病"进行了系统的病原学研究。结果表明,病虾体色发白,反应迟钝,血淋巴液呈浊白色,严重患病白虾血淋巴中的血细胞几乎没有或者极少,代之以大量与血细胞形似的寄生虫;组织病理切片及电镜观察发现,病虾心脏、肝胰腺、肌肉、鳃部等组织寄生有大量单细胞寄生虫,该寄生虫虫体形态特征、显微结构及宿主临床症状与本实验室之前研究报道的感染三疣梭子蟹和锯缘青蟹的血卵涡鞭虫类似;采用已建立的针对血卵涡鞭虫ITS1基因的引物对"白浊病"病虾的血淋巴样品进行PCR检测,可扩增出585bp左右的特异条带。综合以上研究结果,确定该寄生虫为血卵涡鞭虫(Hematodinium sp.)。有关脊尾白虾感染该寄生虫的报道在国内外尚属首次。
In September 2008,＂chalky shrimp disease＂,coined by the local farmer,broke out in ridgetail white prawn Exopalaemon carinicauda in polycultured ponds in the Putuo area,Zhoushan of Zhejiang province,resulting in mass mortality and severe losses.Gross symptoms of the affected shrimp include failure to move with the group,intending to remain near water surface or the sides of ponds,the appearance of khaki,few hemocytes in the blood and a massive population of uninucleate parasites.Diagnostic studies,including bacteria culture,histopathology and ITS DNA sequence analysis,were carried out to identify the pathogeny of the disease.The results showed that no predominant bacteria were isolated from the affected shrimp.Hispathological sections showed that parasites dewelled in the muscle,heart,hepatopancreas,and gills of the affected shrimp,leading to pathological changes such as cells swelling,nucleus condensation,collapse and necroses in these tissues.Under electronic microscopy,masses of the parasites were also observed in the blood and pathological tissues,but no virus particles were observed.Morphological features and internal structures of the parasite and pathological symptoms of host were similar to those of the dinoflagellate Hematodinium sp.previously reported by our lab.A specific amplified product of 585bp was obtained using a set of Hematodinium-specific primers （ITS）.Based on the sequence of this amplified product and comparison with the available sequence of Hematodinium sp.,we confirmed that the parasite was Hematodinium sp..This is the first report on dinoflagellate Hematodinium sp.parasitizing ridgetail white prawn E.carinicauda.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2010年第3期396-402,共7页
Oceanologia Et Limnologia Sinica
基金
浙江省科技厅院所公共服务项目
2008F13002号
浙江省科技厅面上项目
2009C32062号
浙江省自然科学基金项目
Y3080317号
Y3080181号
Y3090402号
关键词
脊尾白虾
白浊病
病原
血卵涡鞭虫
Exopalaemon carinicauda
Milky shrimp disease
Pathogeny
Hematodinium sp.
