摘要
目的以50例中国翼状胬肉患者为研究对象,探讨纤维黏连蛋白(fibronectin,FN)表达水平的改变在翼状胬肉发生中的作用。方法以正常眼结膜组织体为对照,应用半定量RT-PCR方法检测翼状胬肉组织中FN基因mRNA表达水平;应用Western印迹杂交检测FN蛋白表达的改变。结果在50例样本中,40例FN蛋白在翼状胬肉中表达升高,其中包括39例FN mRNA表达水平上调。结论翼状胬肉组织中FN mRNA和蛋白表达水平明显上调,提示其有可能在翼状胬肉的发生中发挥一定的作用。
Objective To study the role of changes in fibronectin （FN） expression levels in development of pterygium in fifty Chinese patients. Methods Using normal conjunctiva as control, the FN gene expression levels of mRNA in pterygium were determined by semi - quantitative RT - PCR. The changes in FN protein expression levels were detected by Western blotting. Results 40 of the 50 patients showed elevation of FN protein expression in pterygium, including increased expression levels of FNmRNA in 39 patients. Conclusion Significant increase takes place in pterygium tissue levels of FN mRNA and protein expressions, suggesting the possible development of pterygium.
出处
《武警医学》
CAS
2010年第4期304-305,共2页
Medical Journal of the Chinese People's Armed Police Force
