摘要
目的检测白细胞介素-4(IL-4)受体α链(IL-4Rα)I50V和Q576R基因多态性与呼吸道合胞病毒(RSV)毛细支气管炎的相关性。方法采用聚合酶链-限制性片段长度多态法检测130例RSV毛细支气管炎患儿和108例健康儿童IL-4Rα/I50V、Q576R基因多态性,应用化学发光法和酶联免疫吸附法检测血清总IgE和鼻咽分泌物(NPS)IL-4R水平。结果病例组II、IV和VV基因型频率为24.6%、51.5%和23.9%,对照组为27.8%、40.7%和31.5%,差异无统计学意义(χ2=2.960,P>0.05);病例组I、V等位基因频率为50.4%、49.6%,对照组为48.1%、51.9%,差异无统计学意义(χ2=0.236,P>0.05),II基因型NPS中IL-4R高于IV和VV基因型(Z=2.031,2.034,P<0.05)。病例组QQ和QR基因型频率为72.3%、27.7%,对照组为64.8%、35.2%,差异无统计学意义(χ2=0.521,P>0.05);病例组Q、R等位基因频率为86.2%、13.8%,对照组为82.4%、17.6%,差异无统计学意义(χ2=1.261,P>0.05),R等位基因携带者NPSIL-4R高于Q等位基因携带者(Z=2.205,P<0.05)。两个位点基因型间血清总IgE差异无统计学意义(F=1.021,t=0.452,P>0.05);基因型频率与病情间亦无关联(χ2=0.322,1.393,P>0.05)。结论 IL-4Rα/50II基因型及576R等位基因携带者与RSV毛细支气管炎NPSIL-4R水平增高相关。
Objective：To investigate the association between interleukin 4 receptor alpha （IL-4Rα） gene polymorphisms and the susceptibility to and the severity of respiratory syncytial virus （RSV） bronchiolitis.Furthermore,to explore the impact of IL-4Rα gene polymorphisms upon IL-4R in nasopharyngeal secretions （NPS） and total serum IgE level.Methods：Genotypes of I50V and Q576R were determined by polymerase chain reaction-restriction fragment length polymorphism analysis（PCR-RFLP） in 130 children with RSV bronchiolitis and 108 controls.Total serum IgE and NPS IL-4R level were assayed with chemiluminescence assay and ELISA,respectively.Results：IL-4Rα/I50V gene polymorphism was present both in case and control group.The genotype frequency was 24.6% （II）,51.5% （IV）,23.9% （VV） in case group,and 27.8% （II）,40.7% （IV）,31.5% （VV） in control group.It showed no significant difference between two groups （χ^2=2.960,P〉0.05）.The allele frequency was 50.4% （I）,49.6% （V） in case group,and 48.1% （I）,51.9% （V） in the controls.There was not significant difference between two groups （χ^2=0.236,P〉0.05）.However,among case group,II genotype was significantly associated with high NPS IL-4R level compared with IV and VV genotypes （Z=2.031,2.034,P〈0.05）.IL-4Rα/Q576R gene polymorphism was present both in case group and control group.The genotype frequency in case group was 70.0% （QQ）,30.0% （QR）,and 64.8% （QQ）,35.2% （QR） in control group.It showed no significant difference between two groups （χ^2=0.521,P〉0.05）.The allele frequency was 86.2% （Q）,13.8% （R） in case group,and 82.4% （Q）,17.6% （R） in the controls.There was not significant difference between two groups （χ^2=1.261,P〉0.05）.R allele was associated with significantly high NPS IL-4R level in case group （Z=2.205,P0.05）.No difference was found in total serum IgE level among three genotypes of I50V （F=1.021,P〉0.05） and between two genotypes of Q576R （t=0.452,P〉0.05）.Furthermore,the genotype frequency of I50V and Q576R did not associated with the severity （χ^2=0.322,1.393,P〉0.05）.Conclusions：II genotype of IL-4Rα/I50V and R allele of IL-4Rα/Q576R are both associated with high NPS IL-4R level in children with RSV bronchiolitis..
出处
《中国优生与遗传杂志》
2010年第7期7-9,共3页
Chinese Journal of Birth Health & Heredity
