摘要
目的 建立人类卵巢癌细胞顺铂耐药细胞株OV1228/cDDP,并对其生物学特性进行检测.方法 采用顺铂浓度梯度递增法,建立人类卵巢癌顺铂耐药细胞株.通过细胞形态学观察、生长曲线和群体倍增时间测定、药物敏感试验、mdr-1和PKC-α基因及蛋白表达水平的测定,来评价OV1228/cDDP的生物学特性.结果 成功建立了OV1228/cDDP耐药细胞株,耐药指数（RI）为5.97,对多柔比星和5-氟尿嘧啶具有一定的交叉耐药性.与OV1228相比,OV1228/cDDP细胞异型性增加、细胞倍增时间延长 mdr-1和PKC-α在基因和蛋白表达水平均明显增高.结论 OV1228/cDDP细胞对顺铂耐药性稳定,并呈现一定的多药耐药特性,为进一步研究耐药逆转途径提供了实验基础.
Objective To establish cisplatin-resistant cell line of human ovarian carcinoma （OV1228/eDDP）and to investigate its biological characteristics.Methods OV1228/cDDP cell line was established by stepwise selection in increasing concentration of cisplatin.Cell morphology,growth curve and population doubling time,mRNA and protein level of mdr-1 and PKC-α were investigated to determine the biological features of OV1228/eDDP cell line.Results The resistant index（RI）of OV1228/cDDP cells was up to 5.97.Cross-resistant of adriamycin（ADM）and 5-Flucytosine（5-Fu）was exhibited.RT-PCR and Western blotting studies showed that the mRNA and protein expression of mdr-1 and PKC-α in the OV1228/cDDP cells were higher than the sensitive cells. Conclusion OV1228/eDDP shows typical multiding resistance phenotypes and might serve as an ideal model for exploring the new route of reversing multidrug resistance.
出处
《肿瘤研究与临床》
CAS
2010年第6期365-367,共3页
Cancer Research and Clinic
基金
江苏省卫生厅面上项目（H200842）
江苏省人事厅“六大人才高峰”项目（08-D-02）
江苏省肿瘤医院重点课题（ZK200701）
关键词
卵巢肿瘤
顺铂
多药耐药
细胞株
Ovarian neoplasms Cisplatin Drug resistance Cell line
