摘要
采用水溶液静态置换法,从中华鲟受精卵发育至96h开始,进行了16周的Pb暴露试验和6周的Pb排放试验,研究了不同浓度Pb2+水溶液(0、0.2、0.8和1.6mg.L-1)对中华鲟幼鱼血液中碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)和肌酸激酶(CK)活力的影响。Pb暴露后结果显示:幼鱼血液中的ALP活力总体表现为随Pb暴露剂量增加而下降的趋势,其中1.6mg.L-1组极显著低于其他各组(P<0.01);幼鱼血液中的LDH和CK活力均表现为随Pb暴露剂量增加而升高的趋势,其中LDH活力只在1.6mg.L-1组显著升高(P<0.05);而CK对Pb浓度则比较敏感,0.8mg.L-1组达到对照组的8倍(P<0.01),1.6mg.L-1组则高达对照组的20倍(P<0.01)。Pb排放后结果显示:幼鱼血液中的ALP活力总体仍表现为随Pb暴露剂量增加而下降的趋势,但各暴露组之间比较差异不显著(P>0.05);幼鱼血液中LDH活力各组之间均无显著差异(P>0.05)。积累和排放对比显示,血液中的ALP活力在0.8和1.6mg.L-1组有所回升但无显著差异(P>0.05);而LDH活力在1.6mg.L-1组极显著降低(P<0.01),表现出明显恢复。初步认为:经1.6mg.L-1Pb2+暴露后的中华鲟幼鱼会发生代谢异常;血液CK变化较ALP和LDH更为明显,是Pb污染的敏感指标。
By using static replacement method, this paper studied the effects of different Pb con- centrations （0, 0. 2, 0. 8, and 1.6 mg · L-1） in water on the activities of alkaline phosphatase （ALP）, lactate dehydrogenase （LDH） and creatine kinase （CK） in the blood of juvenile Chinese sturgeon （Acipenser sinensis）. To Start with the zygote having developed for 96 hours, the juveniles were exposed to Pb for 16 weeks, and then transferred to Pb-free water for 6 weeks. After Pb exposure, the ALP activity in the blood presented a downward trend with increasing Pb concentration, and the decrease was significant when the Pb concentration was 1.6 mg · L-1（P〈 0. 01 ）. The activities of LDH and CK in the blood increased with increasing Pb concentration, and only LDH activity had a significant increase when the Pb concentration was 1.6 mg · L-1（ P 〈0. 05）. The CK activity in the blood was more sensitive to Pb, which was 7 times higher when the Pb concentration was 0. 8 mg · L-1 , and 19 times higher when the Pb concentration was 1.6 mg · L-1 , compared with the control （ P〈0.01 ）. After 6-week recovery in Pb-free water, the ALP activity in the blood still presented a downward trend with increasing Pb concentration, but no significant differences were observed among all treatments （P〉O. 05）. The LDH activity in the blood also had no significant differences among the treatments （P〉0. 05 ）. After transferring to Pb-free water, the ALP activity in the blood had somewhat recovery, whereas the LDH activity recovered significantly. It was considered that after exposure to 1.6 mg· L-1 of Pb, the juvenile A. sinensis could have metabolic abnormality and tissue （liver or muscle） injury. The CK activity in the blood was more sensitive than the ALP and LDH activities, and thus, could be used as a useful index of Pb pollution for fish.
出处
《生态学杂志》
CAS
CSCD
北大核心
2010年第7期1359-1364,共6页
Chinese Journal of Ecology
基金
国家自然科学基金重大项目(30490234)
国家高技术研究发展计划项目(2008AA10Z227)
上海市科研计划项目(08391910300)
上海市长江口中华鲟自然保护区项目(沪鲟保科-20091212003)
中央级公益性科研院所基本科研业务费专项资金资助项目(2007M023,2007M01,2008M01)
关键词
铅
中华鲟
碱性磷酸酶
乳酸脱氢酶
肌酸激酶
lead
Chinese sturgeon （Acipenser sinensis）
alkaline phosphatase
lactate dehydro- genase
creatine kinase.
