摘要
目的：探讨在特定的软骨细胞诱导培养液中，由纤维蛋白（fibrin）修饰的聚乳酸／聚羟基乙酸共聚物（PLGA）杂合支架材料对脂肪源性干细胞成软骨的影响。方法：PLGA支架材料采用快速成形技术制备；PLGA-Fibrin杂和支架材料采用低温冷冻的方法制备。分离兔脂肪源性干细胞，分别种植在未修饰的PLGA支架材料和Fbirin修饰的PLGA上，并在特定软骨诱导液中培养至14d，分别应用SEM观察杂合支架材料的性能；生化方法检测硫酸软骨素的含量；支架材料的亲水性和细胞黏附能力也被检测。通过体内实验对两种支架材料与干细胞复合修复软骨缺损的性能进行形态学染色和评分。结果：体外研究表明，与未修饰的PLGA支架材料相比，Fibfn修饰的PLGA亲水性明显被提高，同时也提高了干细胞在杂合支架材料上的黏附性能和硫酸软骨素的含量。软骨损伤修复研究表明，脂肪源性干细胞与Fibirn修饰的PLGA支架材料复合后，具有良好的修复软骨缺损的功能，其性能明显优于单纯的PLGA材料复合干细胞。结论：Fibim修饰的PLGA支架材料可提高脂肪源性干细胞向软骨细胞分化的能力，促进缺损软骨的再生。
AIM： To investigated the effect of the presence of fibrin in the PLGA scaffold on the differentiation of adipose-derived stem cells （ASCs） into chondrocytes in the chondrogenic media. METHODS： ASCs were prepared by colagenase I digestion of fat from rabbits. The PLGA scaffolds were prepared by LDM technology. The hybrid scaffold was fabricated by a freeze-drying method. Isolated ASCs were cultured in the PLGA without and with fibrin up to 14 days in specific chondrogenic medium. The surface property of the scaffold was observed by SEM. Cell attachment was evaluated, and glycoaminoglycans （GAGs） content was tested by biochemical method. RESULTS： When ASCs were seeded within fibrin modified PLGA scaffolds in vitro, enhanced cellular attachment and differentiation were observed compared to unmodified PLGA scaffold. The study from articular cartilage defect repaired showed that the group from the autologous ASCs seeded on fibrin-PLGA scaffold had better chondrocyte morphology, tissue integration, continuous subchondral bone, and much thicker newly formed cartilage layer as compared with other groups. CONCLUSION： Such modification of PLGA may ultimately enhance the efficacy of tissue engineered scaffolds for cartilage tissue engineering using ASCs.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第8期758-760,共3页
Chinese Journal of Cellular and Molecular Immunology
关键词
脂肪源性干细胞
软骨
纤维蛋白
PLGA
adipose-derived stem cell
cartilage
fibrin polylactic acid/polyglycolic acid
