摘要
目的:探讨孕激素拮抗剂米非司酮对患者子宫内膜癌细胞凋亡、周期及孕激素受体(PR)亚型的影响。方法:患者在米非司酮治疗前及治疗后分别留取标本60例,用流式细胞仪检测组织细胞凋亡率和周期时相的变化,用实时定量PCR检测PR-A mRNA和PR-B mRNA的表达情况。结果:患者经过米非司酮治疗后,癌细胞凋亡率和G0/G1比率升高,S期比率下降,RR-B mRNA表达显著下降,PR-B与PR-A的比例明显降低。结论:米非司酮下调PR-B的表达,使癌细胞阻滞于G1期,促进癌细胞凋亡,抑制子宫内膜癌肿瘤细胞的生长。
Objective:To explore the effect of mifepristone on apoptosis, cell cycle and subtypes of progesterone receptor in patients with endometrial carcinoma.Methods:60 samples were extracted before and after treatment of mifepristone, the rate of cell apoptosis and changes of cell cycle were detected by flow cytometry, the expression levels of PR-A mRNA and PR-B mRNA were detected by real-time quantitative PCR.Results:After treated with mifepristone, the apoptosis rate of cancer cells and ratio of G0/G1 increased, the ratio of S phase decreased, the expression of PR-B mRNA and the ratio of PR-B/PR-A decreased significantly.Conclusion:Mifepristone can down-regulate the expression of PR-B, block cancer cells at G1 phase, promote apoptosis and inhibit the growth of endometrial carcinoma cells.
出处
《中国妇幼保健》
CAS
北大核心
2010年第19期2640-2642,共3页
Maternal and Child Health Care of China
基金
广东省科技厅科研经费资助〔83098〕
关键词
米非司酮
子宫内膜癌
凋亡
细胞周期
孕激素受体
Mifepristone
Endometrial carcinoma
Apoptosis
Cell cycle
Progesterone receptor