摘要
选取黑麦草(Lolium perenne)barti和telstar品系以及高羊茅(Festuca elata)追寻者品系作为相应的受体材料,采用实验室克隆的玉米(Zea mays)抗逆相关转录因子ABP9通过农杆菌介导和基因枪轰击2种方法进行遗传转化,获得再生植株;并对再生植株进行PCR、Southern和Northern杂交分子检测以及抗逆性鉴定。试验结果表明,成功获得ABP9在基因组中整合并表达且抗逆性得到显著提高的转基因黑麦草和高羊茅植株。
Plant expression vectors on which the maize bZIP transcriptional factor ABP9 was under the control of either a constitutive promoter Ubi/35S or an ABA responsive promoter were constructed and transformed into ryegrass and tall fescue via Agrobacterium mediated transformation or particle bombardiment,and the resulting regenerated plants were analyzed by PCR,Southern and Northern blotting for integration and expression of ABP9 and tested for resistance under drought or high salinity conditions.The results showed that transgenic ryegrass and tall fescue plants expressing ABP9 with enhanced stress tolerance have been obtained.
出处
《草业科学》
CAS
CSCD
北大核心
2010年第7期72-77,共6页
Pratacultural Science
基金
"973"国家重点基础研究发展计划项目(2007CB108906)
浙江省重大科技专项国际科技合作项目(2007C14021)
关键词
黑麦草
高羊茅
转基因
ABP9
抗逆性
转录因子
ryegrass
tall fescue
transgenic
ABP9
stress resistance
transcriptional factor
