摘要
讨论了荧光法测定微生物ＴＧａｓｅ的条件，对反应体系中ＭＤＣ的浓度、反应时间、ＤＴＴ的浓度、硫铵的浓度、酶浓度等因素与体系荧光强度的关系进行了探讨，得出３０μｍｏｌ／ＬＭＤＣ１．０ｍＬ，２．２９ｍｇ／ｍＬＤＭＣ１．０ｍＬ，５．５ｍｍｏｌ／ＬＤＴＴ０．３ｍＬ，加入一定量酶样品，３７℃反应３０ｍｉｎ后用１ｍｏｌ／Ｌ硫铵溶液０１ｍＬ灭活，测定体系在３６０ｎｍ激发光下，５３０ｎｍ处的荧光强度值，以此作为定量的依据。
An important assay method has been discussed for microbial transglutaminase regarding dansyl cadaverine incorporation into casein.It is known that the amine,after incorporation into protein by transglutaminase,shows a marked increase of fluorescence accompanied by a slight blue shift.The parameter such as:the concertration of MDC,the reaction time,the concertration of DTT and ammonium sulfate were studied and got better results.The results is:30 μmol/L MDC 1.0 mL,2,29 mg/mL DMC1.0 mL,5.5 mmol/L DTT0.3 mL,37 ℃,30 min.
出处
《郑州粮食学院学报》
1999年第1期32-34,共3页
Journal of Zhengzhou Institute of Technology
关键词
转谷氨酰胺酶
丹磺尸胺
硫酸铵
荧光法
微生物
transglutaminase
monodansyl cadaverine
ammonium sulfate
fluorescence
