摘要
应用携带改造过的长约1．8kb抗虫基因Bt的农杆菌LBA404对苹果品种绿袖、金矮生和长富2号的叶片外植体通过真空渗入接种、共培养和在含50mg／L卡那霉素的培养基上筛选70d，获得对卡那霉素具抗性、存活并产生愈伤组织的外植体为16．7％～373％，其中18．7％～92．9％的外植体经噻重氮苯基脲（TDZ）诱导后，即产生不定芽和形成试管绿苗营养系，营养系经过多聚酶链反应（PCR）测定表明．约有50％以上的营养系具阳性反应，证明Bt基因已被导入苹果。但是，以苹小卷叶蛾幼虫进行虫试检测，抗虫性尚不理想。
Leaf pieces from in vitro-grown shoots of 3 apple cultivars were contaminated byAgrobacterium tumefaciens strain LBA4404 containing the binary vector pBin438 or pB 48. 7 carryingmodefied about 1. 8 kb Bt-gene. The contamination has been done on condition of vaccume Aftercocultivated with the agrobacterium for 3 days and selected by kanamycin for 70 days. The rate of explantsforming callus is 16 % ～37 %. The cxplants With callus have been induced by Thidiazuron (TDZ) to formadventitious buds and to grow out new shoots. The rate of induction is about 18%～ 92% on differentcultivars. According to PCR test, over 50% of regcnerated clones perform positively. It is confirmed thatthe Bt-gene has transformed into apples. However, only a few transformed apples have weak ability ofresistance to tortrix moth (Adoxophyes congrana Walke).
出处
《西北农业学报》
CAS
CSCD
1999年第1期78-81,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金
