摘要
积雪苷片是由积雪草总苷制得的制剂,有每片含总苷为6mg和12mg两种规格,可用于治疗外伤、手术创伤、烧伤、疤痕疙瘩及硬皮病。现有关积雪苷片中总苷的含量测定，依据部颁标准（中药部颁标准WS3．8—2596—97）是测定积雪草总苷由酸水解得到的糖的含量，推算得到总苷含量。但部颁标准的实验操作复杂，过程繁琐且测定误差较大。
An HPLC method was established for the determination of asiaticoside,madecassoside and asiaticoside B in the tablets of Centella triterpenes.A C18 column was used with the mobile phase of acetonitrile-2 mmol/L of β-cyclodextrin（25：75） at the detection wavelength of 205 nm.The calibration curves of asiaticoside,madecassoside and asiaticoside B were linear in ranges of 2-20 μg.The average recoveries were 102.1%,101.6% and 99.1%,with the RSDs of 0.55%,1.38% and 1.59%,respectively.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2010年第7期536-538,共3页
Chinese Journal of Pharmaceuticals
基金
国家"重大新药创制"科技重大专项资助(2009ZX09301007)
关键词
羟基积雪草苷
测定误差
HPLC法
制剂
部颁标准
手术创伤
实验操作
硬皮病
Centella Triterpenes tablets
asiaticoside
madecassoside
asiaticoside B
mobile phase additive method
HPLC
determination
