摘要
利用植原体16S rDNA基因保守序列通用引物R16F2n/R16R2对北京昌平区西峰山村小枣患病植株总DNA进行PCR扩增,结果得到约1.2 kb的特异性DNA片段。对克隆扩增获得DNA片段测序,结果表明,北京昌平地区枣疯病植原体16S rDNA基因片段长1 248 bp,与韩国枣疯病植原体同源性最高,为99.51%。序列同源性比较的结果表明克隆到的植原体归属于榆树黄化组16SrV-C亚组。
By using PCR,the 16S rDNA of phytoplasma of jujube witches broom（JWB） in diseased sample from Beijing was amplified by the universal primer pairs R16F2n/R16R2.Then,the 16S rDNA of 1.2 kb from the diseased sample was obtained.The result of sequencing showed that the JWB strain from Beijing consists of 1 248 bp in 16S rDNA gene,which shared the highest homology（99.51%） with the train of JWB-Korea.The analysis of sequence homology showed that this phytoplasma strain belongs to Elm yellows group（16SrV-C subgroup）.
出处
《湖南农业科学》
2010年第6期13-15,共3页
Hunan Agricultural Sciences
基金
质检公益性行业项目(200810632
200810517)
