摘要
新近对传统的银染技术作出改良，以氨银反应观察精子发生及受精过程中碱性蛋白的更替，以ＡｇＡｓ反应观察精子发生过程中ＮＯＲ、嗜银细胞器、细胞骨架及其它嗜银成份的变化以及皮层反应中嗜银成分的变化。
We modified conventional silver staining technique by applying modified ammoniacal silver reaction.We can observe basic proteins changes during the spermatogenesis and fertilization under light and electronic microscopy.The technique has been employed to study the behavior of nucleolus organizer region of chromosome during the spermatogenesis.A modified silver nitrate technique has also been used to demonstrate argyrophilic organelle,cytoskeletal structure and argyrophilic material in germ cells.It is better to characterize the structure of cortical granules during the cortical reaction using modified silver nitrate reaction.
出处
《动物学杂志》
CAS
CSCD
北大核心
1999年第1期29-33,共5页
Chinese Journal of Zoology
基金
福建省自然科学基金
中国科学院昆明动物研究所细胞与分子进化开放实验室资助
