摘要
目的观察S100A4基因沉默后人胰腺癌Bxpc-3细胞株S100A4和MMP-2的表达及肿瘤细胞迁移和侵袭能力的变化,探讨S100A4基因在肿瘤转移中的作用机制。方法采用RNA干扰技术将S100A4-siRNA转染至人胰腺癌Bxpc-3细胞,采用荧光实时定量PCR和Western免疫印迹方法检测转染前后S100A4和MMP-2的mRNA和蛋白表达;以Transwell小室模型检测转染前后癌细胞迁移和侵袭能力变化。结果转染后S100A4和MMP-2的表达均下调;细胞体外迁移和侵袭能力显著降低(P<0.05)。结论 S100A4基因上调MMP-2的表达是肿瘤细胞具有高侵袭性的机制之一。S100A4基因可以作为胰腺癌基因治疗的一个靶点。
Objective To observe the mRNA and protein expressions of S100A4 and MMP -2,to detect the changes of migrative and invasive abilities of human pancreatic cancer Bxpc-3 cells when S100A4 gene was silenced, and to discuss the role of S100A4 gene on metastasis of pancreatic cancer. Methods Human pancreatic cancer Bxpc-3 cells were transfected in vitro with sequence -specific siRNA targeting S100A4 gene using RNA interference technique. The mRNA and protein expression of S100A4 and MMP-2 before and after transfection were detected by real-time PCR and western- blot respectively. The changes of migrative and invasive abilities were detected by Transwell chamber model. Results The expressions of S100A4 and MMP-2 were both decreased. The migrative and invasive abilities were reduced obviously （P 0.05）. Conclusions S100A4 gene upregulating the expression of MMP -2 is one of the roles of high invasion of tumor cells. S100A4 gene can be considered as one of the target for gene therapy of human pancreatic cancer.
出处
《北京医学》
CAS
2010年第7期562-564,共3页
Beijing Medical Journal
