摘要
目的：探讨造血细胞生长因子（细胞因子）的不同组合对脐血ＣＤ３４＋造血干（祖）细胞的扩增作用。方法：应用吸附单克隆抗体———磁珠分离系统分离纯化脐血ＣＤ３４＋细胞，在液体培养体系中经３组不同组合的细胞因子扩增３周。结果：３组不同组合的细胞因子对ＣＤ３４＋细胞均有明显的扩增作用。其中由ＦＬＴ３配基（ＦＬ）＋干细胞因子（ＳＣＦ）＋白细胞介素（ＩＬ）１＋ＩＬ３的组合扩增效果最好，其细胞总数扩增９０４６７±１３２２３倍，ＣＤ３４＋细胞扩增３７９９±１３２１倍，粒单造血祖细胞（ＣＦＵＧＭ）扩增７２７３±３５９８倍，多向祖细胞（ＣＦＵＧＥＭＭ）扩增３７７６±２３０３倍，总造血集落形成细胞（ＣＦＣｓ）扩增５７９５±２７９６倍。ＣＤ３４＋细胞扩增在第２周达最高值，ＣＦＣｓ在第３周达最高值。结论：由ＦＬ、ＳＣＦ、ＩＬ１及ＩＬ３联合扩增效果最好。ＣＤ３４＋细胞和ＣＦＣｓ分别在第２周、第３周达最高峰，说明其时仍保持造血活性。本实验结果对临床脐血造血干细胞移植选择最佳扩增方案和最佳移植时间有一定的参考价值。
Objective:To explore the effect of different combination of hematopoietic cell growth factors(HGFs)on expanding umbilical cord blood hemopoietic cells in vitro.Methods:CD 34 + cells were purified by using a monoclonal antibody magnetic beads sorting system and then had been expanded with three groups HGFs of different combination in liquid culture system for 21 days.Results:The combination of FLT 3 ligand(FL)with SCF,IL 1 and IL 3 was identified as the most potent of the three groups and total nucleated cells increased by 904 67±132 23 fold,CD 34 + cells by 37 99±13 21 fold,CFU GM by 72 73±35 98 fold,CFU GEMM by 37 76±23 03 fold and CFCs by 57 95±27 96 fold.CD 34 + cells and CFCs reached to their highest level at the 14th day and at the 21st day respectively.Conclusion:The effect of the combination of FL,SCF,IL 1,and IL 3 is the most potent in expanding umbilical cord blood hemopoietic cells with a long activity from the 14th day to 21st day.It may provide a reference for selecting the best scheme and optimal time of cord hemopoietic transplant.
出处
《中日友好医院学报》
1999年第1期3-6,共4页
Journal of China-Japan Friendship Hospital
基金
中日友好医院科研基金
