摘要
目的:探讨参芎化瘀胶囊对脑缺血再灌注损伤的治疗作用及机制。方法:48只雄性SD大鼠随机分成假手术组、模型组、参芎化瘀胶囊高、低剂量组。改良的Pulsineli4血管阻断(4-VO)法制作全脑缺血模型。术后1d应用电镜和光镜观察海马区脑组织形态变化,术后3～7d水迷宫法测试动物学习记忆功能。结果:与假手术组比较,模型组中海马神经元内细胞器、轴索及毛细血管等超微结构明显受损伤、动物搜索安全岛潜伏期延长(P<0.05);与模型组比较,参芎化瘀胶囊组中大鼠脑组织结构损伤程度减轻、搜索安全岛潜伏期缩短(P<0.05);上述变化在高剂量参芎化瘀胶囊组更为明显。结论:参芎化瘀胶囊对脑缺血再灌注损伤有很好的治疗作用,其机制与减轻脑损伤后脑组织超微结构损害有关。
Objective：To investigate the therapeutic effects of Shenxiong Huayu Capsule on cerebral ischemia reperfusion and its underlying mechanism in rats. Method：Male Sprague-Dawley rats（n = 48） were divided randomly into four groups：sham group,model group,low dose of Shenxiong Huayu Capsule treatment group and high dose of Shenxiong Huayu Capsule treatment group. Global cerebral ischemia model was formed by improved four-vessel occlusion as described in the Pulsinelli′s method. Changes in cerebral tissue morphous in hippocampal region were observed with light and electron microscopes 1d after operation. Learning and memory function（Morris water maze） were performed daily from 3d to7d after injury. Result：Compared with the sham group,the neurons,axons and capillaries in the models were damaged,and the latency to find the platform was prolonged（P〈0. 05） . Compared with the model group,the damage of brain tissue in the Capsule treated rats was decreased,latency to find the platform were shortened（P〈0. 05） . The above mentioned improvements were more significant in high dose of Shenxiong Huayu Capsule group. Conclusion：Shenxiong Huayu Capsule has good therapeutic effect on global ischemia-reperfusion injury and the mechanism is related to the attenuating of cerebral ultrastructure damage.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第7期138-141,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省科技厅课题(09276103D-3)
关键词
脑缺血再灌注
超微结构
学习
记忆
参芎化瘀胶囊
cerebral ischemia-reperfusion
ultrastructure
learning
memory
Shenxiong Huayu Capsule
