摘要
目的探讨不同分化程度的鼻咽癌细胞株X线辐射后miR-7表达的变化。方法 2种细胞均分为对照组(未辐射)、2Gy组、6Gy组和10Gy组,X线辐射细胞株,线性二次模型拟合CNE-1、CNE-2细胞存活曲线,Trizol法提取细胞总RNA。茎环法特异性逆转录miR-7,染料法实时定量PCR,以对照组CNE-1细胞为参照样本,ΔΔCt法得出各样品miR-7的相对倍数。结果对照组CNE-2细胞miR-7的量是CNE-1的2.51倍。2Gy组CNE-1细胞miR-7表达量增加,CNE-2细胞则由早期的49.44下降到晚期的0.15。6Gy组两种细胞miR-7含量差别不大。10Gy组CNE-1的miR-7含量高于CNE-2。结论分化程度不同的鼻咽癌细胞经不同剂量的X线辐射后,miR-7表达量各不相同。分化程度不同的鼻咽癌细胞的放射敏感性差异,可能是由调控miR-7表达能力不同造成的。
【Objective】To investigate the expression patterns of miR-7 in variously differentiated nasopharynx cancer cells after radiation. 【Methods】Cells were divided into four groups： control group, 2Gy group, 6Gy group and 10 Gy group. Nasopharynx cancer cell lines CNE1 and CNE2 were irradiated by different dose of X-ray. The cell survival curves were fitted with linear-quadratic model. Total RNAs of cell lines were extracted by Trizol in 2 hours and 10 hours after radiation. The reverse transcription of miR-7 was done by Stem-loop primer. Non-irradiated CNE1 cell was reference sample and relative quantity of other samples was counted by ΔΔCt method after real time PCR used SYBR Green. 【Results】Expression level of miR-7 in CNE-2 was 2.51 times higher than that in CNE-1 of control group. In 2 Gy group expression of MiR-7 in CNE-1 increased; in CNE-2, in earlier period, miR-7 increased to 49.44 and then decreased to 0.15 in later period. There was not distinguishing expression of miR-7 in two cell lines in 6 Gy group. In 10 Gy group, CNE-1 expressed more miR-7 than CNE-2.【Conclusions】 The regulation of miR-7 expression may be the reason of different radiosensitivity of variously differentiated na-sopharynx cancer cell.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第12期1761-1764,共4页
China Journal of Modern Medicine
基金
国家自然科学基金资助项目(No:30670633
No:30973846)
