摘要
目的:建立老年咳喘片中补骨脂素和异补骨脂素的含量测定方法。方法:采用反相高效液相色谱法,色谱柱为KromasilC18(250mm×4.6mm,5μm),流动相为甲醇-水(55:45),流速为1.0mL·min-1,检测波长为246nm。结果:补骨脂素进样量在0.0470～0.9400μg范围内与峰面积积分值呈良好线性关系(r=0.9999),平均回收率为97.70%,RSD=0.89%(n=9);异补骨脂素进样量在0.0460～0.9200μg范围内与峰面积积分值呈良好线性关系(r=0.9999),平均回收率为98.76%,RSD=0.84%(n=9)。结论:本法简便、快速、灵敏、准确,可用于老年咳喘片的质量控制。
OBJECTIVE：To establish a method for content determination of psoralen and isopsoralen in Laonian kechuan tablets.METHODS：RP-HPLC was applied.The separation was performed on Kromasil C18（250 mm×4.6 mm,5μm） column with mobile phase of methanol-water（55：45） at flow rate of 1.0 mL·min^-1.The detection wavelength was set at 246 nm.RESULTS：The linear range of psoralen were 0.047 0～0.940 0 μg with an average recovery of 97.70%（RSD=0.89%,n=9）.The linear range of isopsoralen were 0.046 0～0.920 0 μg with an average recovery of 98.76%（RSD=0.84%,n=9）.CONCLUSION：The method is simple,rapid and accurate for the quality control of Laonian kechuan tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第27期2561-2562,共2页
China Pharmacy
