摘要
目的:了解产生超广谱β-内酰胺酶(ESBLs)的大肠埃希菌耐药表型和AmpC酶基因型。方法:用双纸片扩散法和E-test筛选确认大肠埃希菌产ESBLs和AmpC酶菌株,通过PCR基因扩增对AmpC酶耐药基因型进行确认。结果:68株产生超广谱β-内酰胺酶大肠埃希菌中有1株为AmpC酶。结论:大肠埃希菌是产生ES-BLs的常见菌,同时产生AmpC酶的菌株使其耐药性增强,实验室对其检测和监控非常重要。
Objective： To investigate the drug-resistance phenotype of Escherichia coli which produces extendedspectrum β-lactamases （ESBLs） and AmpC enzymes genotype. Methods： Escherichia coli producing both ESBLs and AmpC strains were screened and detected by double-disc diffusion and E-test. And the AmpC enzymes drug-resistance genotype was confirmed by PCR gene amplification method. Results： There was 1 AmpC strain among 68 strains producing extended-spectrum β-1actamases （ESBLs） Escherichia coli. Conclusion： Escherichia coli is a common bacterium which produces ESBLs and produces strains of AmpC enzymes at the same time to make the drug-resistance increased. It is important to detect and monitor the Escherichia coli in the laboratory.
出处
《山西职工医学院学报》
CAS
2010年第3期1-3,共3页
Journal of Shanxi Medical College for Continuing Education
基金
山西省归国留学人员基金课题(编号200382)
