摘要
目的: 观察К-阿片受体激活对大鼠心肌缺血/再灌注(MI/R)局部心肌中性粒细胞聚集的影响及其相关机制。方法: 将60只成年健康SD大鼠随机分为假手术组、缺血/再灌注(I/R)生理盐水组、К-阿片受体激动剂(U50,488H)组、U50,488H+Nor-BNI组、U50,488H+Wortmannin组及U50,488H+L-精氨酸甲酯(L-NAME)组,每组9~10只大鼠。使心肌局部缺血30 min再灌注3 h后处死动物,检测大鼠心梗面积并按相应试剂盒提供方法检测血清肌酸激酶(CK)、心肌中髓过氧化物酶(MPO)活性、一氧化氮(NO)及心肌和血清中肿瘤坏死因子-α(TNF-α)的水平。结果: 与I/R生理盐水组相比较, U50,488H组的心梗面积(P〈0.05)、血清CK活性(P〈0.05)、心肌MPO(P〈0.05)及心肌和血清TNF-α(P〈0.05)的水平明显下降,同时心肌中NO(P〈0.05)的水平上升;而К-阿片受体的选择性阻断剂(Nor-BNI)、PI3K的选择性抑制剂(Wortmannin)及NO合成酶(NOS)抑制剂(L-NAME)均可消除U50,488H的上述作用(P〈0.05)。结论: U50,488H可通过激活К-阿片受体,发挥对心脏的保护作用。U50,488H的保护作用可能与其激活PI3-激酶, 增加心肌中NO的合成,减少中性粒细胞向I/R损伤心肌的聚集和MI/R诱导的TNF-α生成有关。
AIM: To investigate alterations in neutrophil accumulation and TNF-α levels after activation of kappa (κ)-opioid receptor by U50,488H in ischemia/reperfusion-injured rat hearts and the possible mechanism involved. METHODS: Rats were randomly exposed to sham operation, myocardial ischemia/reperfusion (MI/R) alone, MI/R+U50,488H, MI/R+U50,488H+Wortmannin, and MI/R+U50,488H+L-NAME. RESULTS: Compared with those in MI/R group, U50,488H reduced myocardial infarction area, myocardial myeloperoxidase (MPO) level, serum creatinine kinase (CK) level and serum and myocardial TNF-α production. U50,488H also increased the NOx level in myocardium subjected to I/R injury. All the effects of U50,488H were abolished by Nor-BNI, a selective κ-opioid antagonist, by Wortmannin, a specific PI3K inhibitor; and by L-NAME, a nitric oxide synthase (NOS) inhibitor. CONCLUSION: U50,488H is cardioprotective through the activation of κ-opioid receptor. This protective effect may be associated with the increase in NO production caused by activation of PI3K pathway and the inhibition of neutrophil accumulation and TNF-α introduction caused by U50,488H treatment in myocardium subjected to I/R.
出处
《心脏杂志》
CAS
2010年第4期491-495,共5页
Chinese Heart Journal