摘要
目的：为了研制抗病毒新药，观察了虎杖、黄芪联用抗ＨＳＶ１的药效。方法：用空斑减数实验及中效作用原理对药效进行了分析。结果：在ＨＥｐ２细胞系统中，以虎杖／黄芪＝１（ＥＤ５０）／１（ＥＤ５０）为首选的联用比。①对ＨＳＶ１Ｆ株的直接杀灭，空斑减数率≤８０％时，合用指数＜１．０，有明显的协同作用。②对ＨＳＶ１Ｆ株增殖的抑制，空斑减数率≤６０％时，合用指数＜１．０，有协同作用。③对ＨＳＶ１Ｆ株感染的阻断，空斑减数率从２０％～９０％，合用指数＜１．０，协同作用极为显著，是首选的联用比。结论：两药联用治疗指数达１０３，细胞毒作用并不相加，对于抗ＨＳＶ１而言，是有利的药物联用组合。
Objective:The clinical action of combined use of Rhizoma Polygoni Cuspidati and Radix Astragali on HSV 1 was investigated with a view to developing a new antiviral drug.Method:The action was analyzed by way of plaque reduction assay and median effect principle.Results:In the HEp 2 cell system, if the combination ratio of Rhizoma Polygoni Cuspidati and Radix Astragali was 1(ED 50 )∶1(ED 50 ) then, (1)In directly annihilating HSV 1 F strain, when the plaque reduction rate was 20%～80%,and the combination index was<1.0, there was synergism.(2) In inhibiting the multiplication of HSV 1 F strain, when the plaque reduction rate was 20%～60%, and the combination index was<1.0, there was also synergism. (3)In blockading HSV 1 F strain infection, when the plaque reduction rate was 20%～90% and the combination index was<1.0, there was synergism.So this ratio of 1(ED 50 )∶1(ED 50 ) should be the first choice for combination.Conclusion: The treatment index of the above two Chinese medicinal herbs equals 10 3, and the cytotoxicity is not potentiated,indicating that the combination is helpful as a virucide for HSV 1 F strain.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1999年第3期176-180,共5页
China Journal of Chinese Materia Medica
关键词
虎杖
黄芪
HSV-1F株
HEP-2细胞
中药
药理
Rhizoma Polygoni Cuspidati
Radix Astragali
HSV 1 F strain
HEp 2 cell
median effect principle
