摘要
目的探讨STK15Phe31Ile基因单核苷酸多态与膀胱癌风险的关系。方法采用病例对照研究方法,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测60例膀胱癌患者和60例正常对照者STK15Phe31Ile基因型,比较不同基因型与膀胱癌危险性及其病理特征的关系。结果 STK15Phe31Ile三种基因型(Phe/Phe、Phe/Ile、Ile/Ile)在病例组的分布频率分别为20%、25%和55%,在对照组分别为28.3%、41.6%和30%,分布差异具有统计学意义(P=0.021)。携带STK15Ile/Ile基因型者患膀胱癌的风险比携带STK15Phe/Phe基因型者增加159%(OR=2.59,95%CI=1.019～6.622),此种风险增加在吸烟患者中更为显著(OR=5.33,95%CI=1.282～22.192)。未发现STK15Phe31Ile基因多态与膀胱癌病理特征相关。结论 STK15Phe31Ile基因多态可能是膀胱癌的遗传易感因素。
Objective To detect the association between STK15 Phe31 Ile polymorphism and risk of bladder cancer. Methods In a case-control study,genotypes of STK15 Phe31 Ile were determined by PCR-RLFP in 60 patients with bladder cancer and 60 controls. Their association with bladder cancer risk and pathological outcome were analyzed. The adjusted odds ratios （ORs）and 95% confidence intervals （CIs）were calculated using logistic regression model. Results The frequency of the three genotypes （Phe/Phe, Phe/Ile and Ile/Ile） of STK15 Phe31 Ile in bladder cancer patients was 20%, 25% and 55% respectively, which was significantly different from that in the controls （28.3 %, 41.6 % and 30 % ） （P ： 0. 021 ）. Subjects with the Ile/Ile genotype had an increased risk for the occurrence of bladder cancer compared with those with the Phe/Phe genotype （ OR ： 2.59,95 % CI =1. 019 - 6. 622）. The risk was more significant in the subjects who smoked（ OR ： 5.33,95 % CI ： 1. 282 -22. 192）. No significant association was observed between the STK15 polymorphism and bladder cancer pathological outcome. Conclusion Our study suggestes that STK15 Phe31 Ile polymorphisms are likely to be associated with an increased risk of bladder cancer in Chinese people. Confirmation of this study is needed in a larger case-control population.
出处
《现代泌尿外科杂志》
CAS
2010年第4期277-279,共3页
Journal of Modern Urology
基金
北京市自然科学基金资助项目(No.7052025)
