摘要
目的通过观察不同时间窗被动运动对脑梗死大鼠脑源性神经生长因子（BDNF）及B细胞淋巴瘤／白血病-2基因（Bcl-2）表达的影响，从而探讨康复训练的介入时机及相关治疗机制。方法共选取100只成年健康雄性SD大鼠，并将其随机分为被动训练组、模型组、假手术组及正常对照组，采用线栓法将被动训练组及模型组大鼠制成左侧大脑中动脉栓塞／再灌注模型，假手术组大鼠手术期间不阻断大脑中动脉血流，正常对照组实验期间未给予特殊处理。被动训练组大鼠于脑缺血再灌注后不同时间窗给予被动运动训练。各组大鼠于实验进行14d后取脑组织制成标本，采用PCR技术检测各组大鼠脑梗死灶周边区BDNF及Bcl-2的表达情况。结果各组大鼠均检测到BDNF及Bcl-2阳性表达，并且以被动训练组术后24h、48h亚组BDNF及Bcl-2表达水平相对较高，与其它各组间差异均具有统计学意义（P〈0．05）。结论于脑梗死后24～48h内给予被动运动训练，可促进大鼠脑梗死灶周围区BDNF及Bcl-2表达增强，从而加速受损神经功能恢复。
Objective To observe the effects of passive movement on the functional outcome after occlusion of the middle artery in the brain and reperfusion, and to explore the molecular mechanisms involved. Methods Cerebral infarction models were established in rats using left middle cerebral artery occlusion （MCAO）. The survivors were randomly divided into a passive movement group and a natural recovery group. There was also a sham-operated group and a normal group. Passive movement treatment （twice a day, twenty min per time） was started at different times after reperfusion. The expression of brain-derived neurotrophic factor （BDNF） and B cell lymphoma/leukemia- 2 gene （Bcl-2） were determined using real-time PCRs. Results Expression of BDNF and Bcl-2 was detected a- round the infarction area in both groups. The expression of BDNF and Bcl-2 was highest in the sub-groups where pas- sive movement was begun 24 or 48 h after the operation. Conclusions The expression of BDNF and Bcl-2 in the brain peaks when daily, moderate intensity passive movement is administered beginning 24 to 48 h after reperfusion. Passive movement might have a protective and rehabilitative effect after cerebral infarction.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2010年第7期483-486,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金资助项目（30640010）
