摘要
目的：建立同时测定角花胡颓子中齐墩果酸与熊果酸含量的RP-HPLC法。方法：采用Diamonsil C18（250 mm×4.6mm,5μm）,以甲醇-0.5%乙酸铵（85∶15）为流动相,检测波长为206 nm,流速为1.0 mL·min^-1,柱温30℃。结果：齐墩果酸进样量在0.06-0.36μg（r=0.9995）、熊果酸进样量在0.65-3.9μg（r=0.9997）范围内,线性关系良好,齐墩果酸与熊果酸的平均加样回收率分别为99.2%和102.5%,RSD分别为8.4%和6.0%（n=9）,重复性试验的含量的RSD分别为6.9%和4.2%（n=6）。结论：该法简便、快速、准确,可用于角花胡颓子的质量控制。
Objective：To establish an RP-HPLC for determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth.Methods： The analytical column was Diamonsil C18 column（250 mm×4.5 mm,5 μm）.The mobile phase consisted of methanol-0.5% ammonium acetate（85∶15）.The detective wavelength was 206 nm.The flow rate was 1.0 mL·min^-1 and the column temperature was 30 degrees.Results： Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.36 μg（r = 0.9995） and 0.65-3.9 μg（r=0.9997）.The average recovery of oleanolic acid was 99.2% with relative standard deviation（RSD） of 8.4% and the ursolic acid was102.5%with RSD of 6.0%（n=9）,the reproducibility test of content was 6.9% and 4.2%（n=6）.Conclusion： This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第7期1305-1307,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家支撑计划项目课题(2007BA︱27B06)
关键词
角花胡颓子
齐墩果酸
熊果酸
HPLC
含量测定
Elaeagnus gonyanthes Benth
oleanolic acid
ursolic acid
HPLC
quantitative determination
