摘要
目的:建立同时测定通达颗粒(白芍、红花、黄芩等)中羟基红花黄色素A、芍药苷、阿魏酸和黄芩苷4种化学成分含量的高效液相色谱法。方法:采用Alltech Apollo C18色谱柱(250mm×4.6mm,5μm);以甲醇(A)和质量分数为0.2%的磷酸水溶液(B)作流动相,梯度洗脱;柱温:30℃;流速:1.0mL/min;检测波长:230nm,315nm。结果:羟基红花黄色素A、芍药苷、阿魏酸和黄芩苷的线性范围分别为5.20～104mg/L(r=0.9991),25.6～512mg/L(r=0.9996),7.72～154.4mg/L(r=0.9995)和32.5～650mg/L(r=0.9995),平均加样回收率(n=9)分别为101.1%、99.6%、98.9%和101.9%,RSD分别为2.3%,1.8%,2.6%和1.3%。结论:所建立的方法准确、快速,可用于通达颗粒的质量控制。
AIM：To determine four bioactive components in Tongda Granule （ Radix Paeoniae alba,Flos Carthami,Radix Scutellariale,etc. ）,namely hydroxy safflower yellow A,peoniflorin,ferulic acid,and baicalin. METHODS：The isolation was performed on an Alltech Apollo C18 （250 mm × 4. 6 mm,5 μm） with the mobile phase consisting of methanol-0. 2% （w）phosphoric acid for gradient elution. The flow rate was 1. 0 mL/min and the column temperature was set at 30 ℃. RESULTS：This method was successfully applied to determining four bio-active components in Tongda Granule. The standard curves were linear over the ranges of 5. 20 -104 mg/L for hydroxy safflower yellow A（r =0. 999 1）,25. 6 -512 mg/L for peoniflorin （r =0. 999 6）,7. 72 -154. 4 mg/L for ferulic acid （r =0. 999 5）,32. 5 -650 mg/L for baicalin （r = 0. 999 5）,respectively. The average recoveries were 101. 1% ,99. 6% ,98. 9% and 101. 9% ,RSD were 2. 3% ,1. 8% ,2. 6% and 1. 3% ,respectively. CONCLUSION：The method is simple and can be used as a quality control method for Tongda Granule.
出处
《中成药》
CAS
CSCD
北大核心
2010年第7期1137-1140,共4页
Chinese Traditional Patent Medicine
